C-Kit(+) Cells Isolated from Developing Kidneys Are a Novel Population of Stem Cells with Regenerative Potential

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dc.contributor.author Rangel, Erika Bevilaqua [UNIFESP]
dc.contributor.author Gomes, Samirah Abreu [UNIFESP]
dc.contributor.author Dulce, Raul A.
dc.contributor.author Premer, Courtney
dc.contributor.author Rodrigues, Claudia O.
dc.contributor.author Kanashiro-Takeuchi, Rosimeire Miyuki [UNIFESP]
dc.contributor.author Oskouei, Behzad
dc.contributor.author Carvalho, Decio A.
dc.contributor.author Ruiz, Phillip
dc.contributor.author Reiser, Jochen
dc.contributor.author Hare, Joshua M.
dc.date.accessioned 2016-01-24T14:32:01Z
dc.date.available 2016-01-24T14:32:01Z
dc.date.issued 2013-08-01
dc.identifier http://dx.doi.org/10.1002/stem.1412
dc.identifier.citation Stem Cells. Hoboken: Wiley-Blackwell, v. 31, n. 8, p. 1644-1656, 2013.
dc.identifier.issn 1066-5099
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/36555
dc.description.abstract The presence of tissue specific precursor cells is an emerging concept in organ formation and tissue homeostasis. Several progenitors are described in the kidneys. However, their identity as a true stem cell remains elusive. Here, we identify a neonatal kidney-derived c-kit(+) cell population that fulfills all of the criteria as a stem cell. These cells were found in the thick ascending limb of Henle's loop and exhibited clonogenicity, self-renewal, and multipotentiality with differentiation capacity into mesoderm and ectoderm progeny. Additionally, c-kit(+) cells formed spheres in nonadherent conditions when plated at clonal density and expressed markers of stem cells, progenitors, and differentiated cells. Ex vivo expanded c-kit(+) cells integrated into several compartments of the kidney, including tubules, vessels, and glomeruli, and contributed to functional and morphological improvement of the kidney following acute ischemia-reperfusion injury in rats. Together, these findings document a novel neonatal rat kidney c-kit(+) stem cell population that can be isolated, expanded, cloned, differentiated, and used for kidney repair following acute kidney injury. These cells have important biological and therapeutic implications. STEM Cells 2013;31:1644-1656 en
dc.description.sponsorship James and Esther King Florida Biomedical Research Program
dc.description.sponsorship National Institutes of Health
dc.format.extent 1644-1656
dc.language.iso eng
dc.publisher Wiley-Blackwell
dc.relation.ispartof Stem Cells
dc.rights Acesso aberto
dc.subject C-kit en
dc.subject Kidney stem cells en
dc.subject Clonogenicity en
dc.subject Self-renewal en
dc.subject Multipotentiality en
dc.subject Regenerative potential en
dc.title C-Kit(+) Cells Isolated from Developing Kidneys Are a Novel Population of Stem Cells with Regenerative Potential en
dc.type Artigo
dc.rights.license http://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.contributor.institution Univ Miami
dc.contributor.institution Albert Einstein Hosp
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ Miami, Leonard M Miller Sch Med, Interdisciplinary Stem Cell Inst, Miami, FL 33136 USA
dc.description.affiliation Univ Miami, Leonard M Miller Sch Med, Dept Mol & Cellular Pharmacol, Miami, FL 33136 USA
dc.description.affiliation Univ Miami, Leonard M Miller Sch Med, Dept Surg, Miami, FL 33136 USA
dc.description.affiliation Univ Miami, Leonard M Miller Sch Med, Dept Pathol, Miami, FL 33136 USA
dc.description.affiliation Univ Miami, Leonard M Miller Sch Med, Div Nephrol & Hypertens, Miami, FL 33136 USA
dc.description.affiliation Univ Miami, Leonard M Miller Sch Med, Div Cardiol, Dept Med, Miami, FL 33136 USA
dc.description.affiliation Albert Einstein Hosp, Soc Beneficente Albert Einstein, São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Med, Div Nephrol, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Med, Div Nephrol, São Paulo, Brazil
dc.description.sponsorshipID James and Esther King Florida Biomedical Research Program: 1KD07-33958
dc.description.sponsorshipID National Institutes of Health: HL107110
dc.description.sponsorshipID National Institutes of Health: AG025017
dc.identifier.doi 10.1002/stem.1412
dc.description.source Web of Science
dc.identifier.wos WOS:000323418700018



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