C-Kit(+) Cells Isolated from Developing Kidneys Are a Novel Population of Stem Cells with Regenerative Potential

C-Kit(+) Cells Isolated from Developing Kidneys Are a Novel Population of Stem Cells with Regenerative Potential

Author Rangel, Erika Bevilaqua Autor UNIFESP Google Scholar
Gomes, Samirah Abreu Autor UNIFESP Google Scholar
Dulce, Raul A. Google Scholar
Premer, Courtney Google Scholar
Rodrigues, Claudia O. Google Scholar
Kanashiro-Takeuchi, Rosimeire Miyuki Autor UNIFESP Google Scholar
Oskouei, Behzad Google Scholar
Carvalho, Decio A. Google Scholar
Ruiz, Phillip Google Scholar
Reiser, Jochen Google Scholar
Hare, Joshua M. Google Scholar
Institution Univ Miami
Albert Einstein Hosp
Universidade Federal de São Paulo (UNIFESP)
Abstract The presence of tissue specific precursor cells is an emerging concept in organ formation and tissue homeostasis. Several progenitors are described in the kidneys. However, their identity as a true stem cell remains elusive. Here, we identify a neonatal kidney-derived c-kit(+) cell population that fulfills all of the criteria as a stem cell. These cells were found in the thick ascending limb of Henle's loop and exhibited clonogenicity, self-renewal, and multipotentiality with differentiation capacity into mesoderm and ectoderm progeny. Additionally, c-kit(+) cells formed spheres in nonadherent conditions when plated at clonal density and expressed markers of stem cells, progenitors, and differentiated cells. Ex vivo expanded c-kit(+) cells integrated into several compartments of the kidney, including tubules, vessels, and glomeruli, and contributed to functional and morphological improvement of the kidney following acute ischemia-reperfusion injury in rats. Together, these findings document a novel neonatal rat kidney c-kit(+) stem cell population that can be isolated, expanded, cloned, differentiated, and used for kidney repair following acute kidney injury. These cells have important biological and therapeutic implications. STEM Cells 2013;31:1644-1656
Keywords C-kit
Kidney stem cells
Regenerative potential
Language English
Sponsor James and Esther King Florida Biomedical Research Program
National Institutes of Health
Grant number James and Esther King Florida Biomedical Research Program: 1KD07-33958
National Institutes of Health: HL107110
National Institutes of Health: AG025017
Date 2013-08-01
Published in Stem Cells. Hoboken: Wiley-Blackwell, v. 31, n. 8, p. 1644-1656, 2013.
ISSN 1066-5099 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 1644-1656
Origin http://dx.doi.org/10.1002/stem.1412
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000323418700018
URI http://repositorio.unifesp.br/handle/11600/36555

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