Lateral hypothalamic serotonin is not stimulated during central leptin hypophagia

Lateral hypothalamic serotonin is not stimulated during central leptin hypophagia

Author Telles, Monica Marques Autor UNIFESP Google Scholar
Silva, Thais Girão da Autor UNIFESP Google Scholar
Watanabe, Regina Lucia Harumi Autor UNIFESP Google Scholar
Andrade, Iracema Senna de Autor UNIFESP Google Scholar
Estadella, Debora Autor UNIFESP Google Scholar
Nascimento, Claudia Maria Oller do Autor UNIFESP Google Scholar
Oyama, Lila Missae Autor UNIFESP Google Scholar
Ribeiro, Eliane Beraldi Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Whether leptin targets the hypothalamic serotonergic system to inhibit food intake is not established. We examined the effect of a short-term i.c.v. leptin treatment on serotonin microdialysate levels in rat lateral hypothalamus. Adipose tissue gene expression was also evaluated.Male rats received four daily injections of leptin (5 mu g) or vehicle (with pair-feeding to leptin-induced intake) and a fifth injection during collection of LH microdialysates. We found that serotonin and 5-HIAA levels were not affected by the leptin pre-treatment, as basal levels were similar between the leptin and the pair-fed group. These levels remained unaltered after the acute leptin injection.For gene expression studies, rats were pre-treated with five daily injections of either leptin (5 mu g) or vehicle (with either pair-feeding or ad libitum intake). mRNA levels of resistin, adiponectin, lipoprotein lipase, and PPAR-gamma were unaltered by either leptin or pair-feeding. Leptin gene expression was significantly reduced by leptin but not by pair-feeding, in both the retroperitoneal (- 74%) and the epididymal (- 99%) depots while no differences were observed in the subcutaneous depot.The observations confirmed the absence of an acute stimulatory effect of central leptin on serotonin release in the lateral hypothalamus and showed that the pre-treatment with leptin failed to modify this pattern. This indicates that components of the serotonergic system are probably not directly affected by leptin. Additionally, the central effect of leptin was able to downregulate its own adipose tissue gene expression in a depot-specific manner while other adipokine genes were not affected. (C) 2013 Elsevier B.V. All rights reserved.
Keywords Adipokines
Food intake
In vivo microdialysis
Leptin
Serotonin
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2013-06-10
Published in Regulatory Peptides. Amsterdam: Elsevier B.V., v. 184, p. 75-80, 2013.
ISSN 0167-0115 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 75-80
Origin http://dx.doi.org/10.1016/j.regpep.2013.03.027
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000320220700011
URI http://repositorio.unifesp.br/handle/11600/36424

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