Flavonone treatment reverses airway inflammation and remodelling in an asthma murine model

Flavonone treatment reverses airway inflammation and remodelling in an asthma murine model

Author Toledo, A. C. Google Scholar
Sakoda, C. P. P. Autor UNIFESP Google Scholar
Perini, A. Google Scholar
Pinheiro, N. M. Google Scholar
Magalhaes, R. M. Google Scholar
Grecco, S. Autor UNIFESP Google Scholar
Tiberio, I. F. L. C. Google Scholar
Camara, N. O. Google Scholar
Martins, M. A. Google Scholar
Lago, J. H. G. Autor UNIFESP Google Scholar
Prado, C. M. Autor UNIFESP Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract Background and Purpose Asthma is an inflammatory disease that involves airway hyperresponsiveness and remodelling. Flavonoids have been associated to anti-inflammatory and antioxidant activities and may represent a potential therapeutic treatment of asthma. Our aim was to evaluate the effects of the sakuranetin treatment in several aspects of experimental asthma model in mice. Experimental Approach Male BALB/c mice received ovalbumin (i.p.) on days 0 and 14, and were challenged with aerolized ovalbumin 1% on days 24, 26 and 28. Ovalbumin-sensitized animals received vehicle (saline and dimethyl sulfoxide, DMSO), sakuranetin (20mg kg1 per mice) or dexamethasone (5mg kg1 per mice) daily beginning from 24th to 29th day. Control group received saline inhalation and nasal drop vehicle. On day 29, we determined the airway hyperresponsiveness, inflammation and remodelling as well as specific IgE antibody. RANTES, IL-5, IL-4, Eotaxin, IL-10, TNF-, IFN- and GMC-SF content in lung homogenate was performed by Bioplex assay, and 8-isoprostane and NF-kB activations were visualized in inflammatory cells by immunohistochemistry. Key Results We have demonstrated that sakuranetin treatment attenuated airway hyperresponsiveness, inflammation and remodelling; and these effects could be attributed to Th2 pro-inflammatory cytokines and oxidative stress reduction as well as control of NF-kB activation. Conclusions and Implications These results highlighted the importance of counteracting oxidative stress by flavonoids in this asthma model and suggest sakuranetin as a potential candidate for studies of treatment of asthma.
Keywords sakuranetin
experimental asthma model
airway remodelling
cytokines
oxidative stress
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2013-04-01
Published in British Journal of Pharmacology. Hoboken: Wiley-Blackwell, v. 168, n. 7, p. 1736-1749, 2013.
ISSN 0007-1188 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 1736-1749
Origin http://dx.doi.org/10.1111/bph.12062
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000316262500016
URI http://repositorio.unifesp.br/handle/11600/36116

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