The effect of imatinib mesylate on the proliferation, invasive ability, and radiosensitivity of retinoblastoma cell lines

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dc.contributor.author Moura, L. R. de
dc.contributor.author Marshall, J-C
dc.contributor.author Di Cesare, S.
dc.contributor.author Fernandes, B. F.
dc.contributor.author Antecka, E.
dc.contributor.author Burnier, M. N.
dc.date.accessioned 2016-01-24T14:30:50Z
dc.date.available 2016-01-24T14:30:50Z
dc.date.issued 2013-01-01
dc.identifier http://dx.doi.org/10.1038/eye.2012.231
dc.identifier.citation Eye. London: Nature Publishing Group, v. 27, n. 1, p. 92-99, 2013.
dc.identifier.issn 0950-222X
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/35657
dc.description.abstract Purpose Our aim was to evaluate the potential effect of imatinib mesylate (IM), a small molecule that specifically inhibits the tyrosine quinase receptors, on the proliferation and invasive abilities of two human retinoblastoma (Rb) cell lines. Furthermore, the ability of IM to radiosensitize Rb cells was evaluated. the potential targets of IM (C-kit, PDGRF-alpha and -beta, and c-Abl) were also investigated in these cell lines.Methods Two human Rb cell lines (WERI-RB-1 and Y79) were cultured under normal growth conditions. An MTT-based proliferation assay and a Matrigel invasion assay were performed with and without exposure to 10 mu M of IM. the cells were also irradiated with graded dosages of 0, 2, 4, 6, 8, and 10 Gy with and without IM and their proliferations rates were analyzed. Western blot and immunocytochemical analysis of cytospins were performed to evaluate the expression of C-kit, PDGRF-alpha and -beta, and c-Abl.Results When IM was added to both cell lines a statistically significant (P<0.05) reduction in proliferation and invasive ability were observed. Exposure to IM also significantly increased the radiosensitivity of both Rb cell lines. the c-Abl expression was strongly positive, PDGRF-alpha and -beta expression were also positive but the C-kit expression was negative in both cell lines.Conclusions These results indicate that Gleevec may be useful as an adjuvant treatment in Rb patients, specially those considered for radiation therapy. Eye (2013) 27, 92-99; doi:10.1038/eye.2012.231; published online 16 November 2012 en
dc.description.sponsorship PAAO
dc.description.sponsorship McGill University
dc.format.extent 92-99
dc.language.iso eng
dc.publisher Nature Publishing Group
dc.relation.ispartof Eye
dc.rights Acesso aberto
dc.subject retinoblastoma en
dc.subject cell lines en
dc.subject imatinib mesylate en
dc.subject radiation en
dc.subject c-kit en
dc.subject tyrosine kinase en
dc.title The effect of imatinib mesylate on the proliferation, invasive ability, and radiosensitivity of retinoblastoma cell lines en
dc.type Artigo
dc.contributor.institution McGill Univ
dc.contributor.institution Henry C Witelson Ocular Pathol Lab
dc.contributor.institution Inst Brasileiro Oftalmol
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation McGill Univ, Ctr Hlth, Dept Ophthalmol & Pathol, Montreal, PQ, Canada
dc.description.affiliation Henry C Witelson Ocular Pathol Lab, Montreal, PQ, Canada
dc.description.affiliation Inst Brasileiro Oftalmol, Rio de Janeiro, Brazil
dc.description.affiliation Universidade Federal de São Paulo UNIFESP EPM, Dept Ophthalmol, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo UNIFESP EPM, Dept Ophthalmol, São Paulo, Brazil
dc.identifier.doi 10.1038/eye.2012.231
dc.description.source Web of Science
dc.identifier.wos WOS:000313557200013



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