Leukocytes from wheezing infants release lower amounts of IL-12 and IFN-gamma compared to non-wheezing infants

Leukocytes from wheezing infants release lower amounts of IL-12 and IFN-gamma compared to non-wheezing infants

Author Falcai, Angela Google Scholar
Soeiro Pereira, Paulo Vitor Google Scholar
Kubo, Christina Arslanian Google Scholar
Rullo, Vera Google Scholar
Errante, Paolo Rugero Google Scholar
Sole, Dirceu Autor UNIFESP Google Scholar
Condino-Neto, Antonio Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Santos Med Sch
Abstract Objective This study investigated environmental endotoxin exposure during early life, sensitization to aeroallergens, the production of cytokines by LPS-stimulated leukocytes, and the development of a wheezing phenotype in a prospective cohort of infants with high risk of developing allergic diseases. Materials and Methods Eighty-four infants were followed from birth until 30 months of age. We assessed endotoxin concentration in house dust of their homes during the first 6 months of life. At age 30 months they were clinically evaluated to determine the development of wheezing and other clinical events, were skin prick tested, and had blood samples collected for the evaluation of cytokine release by LPS-stimulated peripheral blood mononuclear cells (PBMC). Results the level of endotoxin exposure during early life was not associated with development of a wheezing phenotype. On the other hand a higher incidence of respiratory infections occurred among recurrent wheezing (RW) infants. PBMC from RW children exposed to higher levels of environmental endotoxin (above 50?EU/mg) released less Interleukin (IL)-12p70 and IFN-? compared to the non-RW group. TNF-a, IL-10, IL-4, IL-5, and IL17 production by LPS-stimulated PBMC from RW and non-RW children was equivalent in both groups of environmental endotoxin exposure. Conclusion in this prospective cohort of infants with high risk of developing allergic diseases we observed that RW and non-RW children were exposed to similar levels of endotoxin early in life. LPS-stimulated PBMC from RW infants exposed to higher levels of endotoxin released significantly less IL-12 and IFN-? compared to non-RW infants. Pediatr Pulmonol. 2012. 47:10541060. (C) 2012 Wiley Periodicals, Inc.
Keywords cytokines
endotoxin
LPS
asthma
infections
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 2006/6529834
FAPESP: 2007/55779-1
Date 2012-10-01
Published in Pediatric Pulmonology. Hoboken: Wiley-Blackwell, v. 47, n. 11, p. 1054-1060, 2012.
ISSN 8755-6863 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 1054-1060
Origin http://dx.doi.org/10.1002/ppul.22598
Access rights Closed access
Type Article
Web of Science ID WOS:000310032500003
URI http://repositorio.unifesp.br/handle/11600/35339

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