Giant Amazonian fish pirarucu (Arapaima gigas): Its viscera as a source of thermostable trypsin

Giant Amazonian fish pirarucu (Arapaima gigas): Its viscera as a source of thermostable trypsin

Author Freitas-Junior, Augusto C. V. Google Scholar
Costa, Helane M. S. Google Scholar
Icimoto, Marcelo Yudi Autor UNIFESP Google Scholar
Hirata, Izaura Yoshico Autor UNIFESP Google Scholar
Marcondes, Marcelo Autor UNIFESP Google Scholar
Carvalho, Luiz B. Google Scholar
Oliveira, Vitor Autor UNIFESP Google Scholar
Bezerra, Ranilson S. Google Scholar
Institution Universidade Federal de Pernambuco (UFPE)
Universidade Federal de São Paulo (UNIFESP)
Abstract A trypsin was purified from pyloric caeca of pirarucu (Arapaima gigas). the effect of metal ions and protease inhibitors on its activity and its physicochemical and kinetic properties, as well its N-terminal sequence, were determined. A single band (28.0 kDa) was observed by SDS-PAGE. Optimum pH and temperature were 9.0 and 65 degrees C, respectively. the enzyme was stable after incubation for 30 min in a wide pH range (6.0-11.5) and at 55 degrees C. the kinetic parameters K-m, k(cat) and k(cat)/K-m were 0.47 +/- 0.042 mM, 1.33 s(-1) and 2.82 s(-1) mM(-1), respectively, using BApNA as substrate. This activity was shown to be very sensitive to some metal ions, such as Fe2+, Hg2+, Zn2+, Al3+, Pb2+, and was highly inhibited by trypsin inhibitors. the trypsin N-terminal sequence IVGGYECPRNSVPYQ was found. the features of this alkaline peptidase suggest that it may have potential for industrial applications (e.g. food and detergent industries). (C) 2012 Elsevier B.V. All rights reserved.
Keywords Arapaima gigas
Air-breathing fish
Fish processing waste
Digestive enzymes
Proteases
Trypsin purification
Language English
Sponsor Financiadora de Estudos e Projetos (FINEP/RECAR-CINE)
Ministerio da Aquicultura e Pesca (MAP)
Empresa brasileira de pesquisa agropecuaria (Embrapa)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundacao de Apoio a Ciencia e Tecnologia do Estado de Pernambuco (FACEPE)
Petroleo do Brasil S/A (PETROBRAS)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2012-08-15
Published in Food Chemistry. Oxford: Elsevier B.V., v. 133, n. 4, p. 1596-1602, 2012.
ISSN 0308-8146 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 1596-1602
Origin http://dx.doi.org/10.1016/j.foodchem.2012.02.056
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000303847400065
URI http://repositorio.unifesp.br/handle/11600/35176

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