GENERATION of NITRIC OXIDE and REACTIVE OXYGEN SPECIES BY NEUTROPHILS and MONOCYTES FROM SEPTIC PATIENTS and ASSOCIATION WITH OUTCOMES

GENERATION of NITRIC OXIDE and REACTIVE OXYGEN SPECIES BY NEUTROPHILS and MONOCYTES FROM SEPTIC PATIENTS and ASSOCIATION WITH OUTCOMES

Author Santos, Sidneia Sousa Autor UNIFESP Google Scholar
Brunialti, Milena Karina Coló Autor UNIFESP Google Scholar
Rigato, Otelo Autor UNIFESP Google Scholar
Machado, Flavia Ribeiro Autor UNIFESP Google Scholar
Silva, Eliezer Autor UNIFESP Google Scholar
Salomao, Reinaldo Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Hosp Albert Einstein
Hosp Sirio Libanes
Abstract In this study, our aims were to evaluate the reactive oxygen species (ROS) and nitric oxide (NO) generation by monocytes and neutrophils from septic patients and to correlate their levels with clinical outcomes. Forty-nine septic patients and 19 healthy volunteers were enrolled in the study. the ROS and NO production was quantified in monocytes and neutrophils in whole blood by flow cytometry, constitutively, and after stimulation with Staphylococcus aureus and Pseudomonas aeruginosa. Nitric oxide production by monocytes was higher in septic patients compared with healthy volunteers for all conditions and by neutrophils at baseline, and ROS generation in monocytes and neutrophils was higher in septic patients than in healthy volunteers for all conditions. Nitric oxide production by monocytes and neutrophils was decreased at day 7 compared with that at admission (day 0) in survivors at baseline and after stimulation with S. aureus. Reactive oxygen species production by the monocytes and neutrophils was decreased in survivors at day 7 compared with day 0 under all conditions, except by neutrophils at baseline. No difference was found in NO and ROS generation by monocytes and neutrophils between day 7 and day 0 in nonsurvivors. Generation of NO and ROS by neutrophils and monocytes is increased in septic patients, and their persistence is associated with poor outcome.
Keywords NO
oxidative burst
phagocytes
sepsis
septic shock
flow cytometry
Language English
Date 2012-07-01
Published in Shock. Philadelphia: Lippincott Williams & Wilkins, v. 38, n. 1, p. 18-23, 2012.
ISSN 1073-2322 (Sherpa/Romeo, impact factor)
Publisher Lippincott Williams & Wilkins
Extent 18-23
Origin http://dx.doi.org/10.1097/SHK.0b013e318257114e
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000305501100004
URI http://repositorio.unifesp.br/handle/11600/35015

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