Baccharis dracunculifolia, the main source of green propolis, exhibits potent antioxidant activity and prevents oxidative mitochondrial damage

Baccharis dracunculifolia, the main source of green propolis, exhibits potent antioxidant activity and prevents oxidative mitochondrial damage

Author Guimaraes, Natalia S. S. Google Scholar
Mello, Joyce C. Google Scholar
Paiva, Juliana S. Google Scholar
Bueno, Paula C. P. Google Scholar
Berretta, Andresa A. Google Scholar
Torquato, Ricardo José Soares Autor UNIFESP Google Scholar
Nantes, Iseli L. Google Scholar
Rodrigues, Tiago Google Scholar
Institution Univ Mogi das Cruzes UMC
Univ Estadual Paulista UNESP
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Universidade Federal do ABC (UFABC)
Abstract Baccharis dracunculifolia DC (Asteraceae) is the main botanical source used by honeybees to produce Brazilian green propolis whose hepatoprotective properties have been already described. in this work we investigated the protective effects of the glycolic extract of B. dracunculifolia (GEBd) against oxidative stress in isolated rat liver mitochondria (RLM). the GEBd was prepared by fractionated percolation using propylene glycol as solvent. the total phenols and flavonoids, which are substances with recognized antioxidant action, were quantified in GEBd and the phytochemical analysis was carried out by HPLC. GEBd exhibited significant scavenger activity towards DPPH radicals and superoxide anions in a concentration-dependent manner, and also a Fe2+ chelating activity. GEBd decreased the basal H2O2 generation and the Fe2+- or t-BuOOH-induced ROS production in isolated mitochondria. Lipid oxidation of mitochondrial membranes, protein thiol groups and GSH oxidation were also prevented by GEBd. This shows that B. dracunculifolia exhibit potent antioxidant activity protecting liver mitochondria against oxidative damage and such action probably contribute to the antioxidant and hepatoprotective effects of green propolis. (C) 2011 Elsevier B.V. All rights reserved.
Keywords Baccharis dracunculifolia
Antioxidant
Free radicals
Hepatoprotection
Mitochondria
Oxidative stress
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 2008/01724-4
FAPESP: 2008/07246-7
CNPq: 301672/2009-1
CNPq: 136255/2009-4
Date 2012-03-01
Published in Food and Chemical Toxicology. Oxford: Pergamon-Elsevier B.V., v. 50, n. 3-4, p. 1091-1097, 2012.
ISSN 0278-6915 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 1091-1097
Origin http://dx.doi.org/10.1016/j.fct.2011.11.014
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000303284600091
URI http://repositorio.unifesp.br/handle/11600/34633

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