The Natural Cell-Penetrating Peptide Crotamine Targets Tumor Tissue in Vivo and Triggers a Lethal Calcium-Dependent Pathway in Cultured Cells

The Natural Cell-Penetrating Peptide Crotamine Targets Tumor Tissue in Vivo and Triggers a Lethal Calcium-Dependent Pathway in Cultured Cells

Author Nascimento, Fabio D. Google Scholar
Sancey, Lucie Google Scholar
Pereira, Alexandre Google Scholar
Rome, Claire Google Scholar
Oliveira, Vitor Autor UNIFESP Google Scholar
Oliveira, Eduardo B. Google Scholar
Nader, Helena B. Autor UNIFESP Google Scholar
Yamane, Tetsuo Google Scholar
Kerkis, Irina Google Scholar
Tersariol, Ivarne L. S. Google Scholar
Coll, Jean-Luc Google Scholar
Hayashi, Mirian A. F. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Bandeirante São Paulo UNIBAN
Univ Grenoble 1
Inst Butantan
Universidade de São Paulo (USP)
Univ Mogi das Cruzes
Abstract Our goal was to demonstrate the in vivo tumor specific accumulation of crotamine, a natural peptide from the venom of the South American rattlesnake Crotalus durissus terrificus, which has been characterized by our group as a cell penetrating peptide with a high specificity for actively proliferating cells and with a concentration-dependent cytotoxic effect. Crotamine cytotoxicity has been shown to be dependent on the disruption of lysosomes and subsequent activation of intracellular proteases. in this work, we show that the cytotoxic effect of crotamine also involves rapid intracellular calcium release and loss of mitochondrial membrane potential as observed in real time by confocal microscopy. the intracellular calcium overload induced by crotamine was almost completely blocked by thapsigargin. Microfluorimetry assays confirmed the importance of internal organelles, such as lysosomes and the endoplasmic reticulum, as contributors for the intracellular calcium increase, as well as the extracellular medium. Finally, we demonstrate here that crotamine injected intraperitoneally can efficiently target remote subcutaneous tumors engrafted in nude mice, as demonstrated by a noninvasive optical imaging procedure that permits in vivo real-time monitoring of crotamine uptake into tumor tissue. Taken together, our data indicate that the cytotoxic peptide crotamine can be used potentially for a dual purpose: to target and detect growing tumor tissues and to selectively trigger tumor cell death.
Keywords mitochondria
cellular uptake
tumor-specific cell-penetrating peptides
actively proliferating cells
intracellular calcium
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
University Joseph Fourier of Grenoble
Date 2012-02-01
Published in Molecular Pharmaceutics. Washington: Amer Chemical Soc, v. 9, n. 2, p. 211-221, 2012.
ISSN 1543-8384 (Sherpa/Romeo, impact factor)
Publisher Amer Chemical Soc
Extent 211-221
Access rights Closed access
Type Article
Web of Science ID WOS:000300474900002

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