First Description of Natural and Experimental Conjugation between Mycobacteria Mediated by a Linear Plasmid

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dc.contributor.author Rabello, Michelle Christiane da Silva [UNIFESP]
dc.contributor.author Matsumoto, Cristianne Kayoko [UNIFESP]
dc.contributor.author Almeida, Luiz Gonzaga Paula de
dc.contributor.author Carmen Menendez, Maria
dc.contributor.author Oliveira, Rosangela Siqueira de
dc.contributor.author Silva, Rosa Maria [UNIFESP]
dc.contributor.author Jesus Garcia, Maria
dc.contributor.author Leão, Sylvia Cardoso [UNIFESP]
dc.date.accessioned 2016-01-24T14:17:48Z
dc.date.available 2016-01-24T14:17:48Z
dc.date.issued 2012-01-03
dc.identifier http://dx.doi.org/10.1371/journal.pone.0029884
dc.identifier.citation Plos One. San Francisco: Public Library Science, v. 7, n. 1, 8 p., 2012.
dc.identifier.issn 1932-6203
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/34522
dc.description.abstract Background: in a previous study, we detected the presence of a Mycobacterium avium species-specific insertion sequence, IS1245, in Mycobacterium kansasii. Both species were isolated from a mixed M. avium-M. kansasii bone marrow culture from an HIV-positive patient. the transfer mechanism of this insertion sequence to M. kansasii was investigated here.Methodology/Principal Findings: A linear plasmid (pMA100) was identified in all colonies isolated from the M. avium-M. kansasii mixed culture carrying the IS1245 element. the linearity of pMA100 was confirmed. Other analyses suggested that pMA100 contained a covalently bound protein in the terminal regions, a characteristic of invertron linear replicons. Partial sequencing of pMA100 showed that it bears one intact copy of IS1245 inserted in a region rich in transposase-related sequences. These types of sequences have been described in other linear mycobacterial plasmids. Mating experiments were performed to confirm that pMA100 could be transferred in vitro from M. avium to M. kansasii. pMA100 was transferred by in vitro conjugation not only to the M. kansasii strain from the mixed culture, but also to two other unrelated M. kansasii clinical isolates, as well as to Mycobacterium bovis BCG Moreau.Conclusions/Significance: Horizontal gene transfer (HGT) is one of most important mechanisms leading to the evolution and diversity of bacteria. This work provides evidence for the first time on the natural occurrence of HGT between different species of mycobacteria. Gene transfer, mediated by a novel conjugative plasmid, was detected and experimentally reproduced. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Cooperacion Interuniversitaria UAM-Banco Santander con America Latina (CEAL), UAM, Spain
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorship Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent 8
dc.language.iso eng
dc.publisher Public Library Science
dc.relation.ispartof Plos One
dc.rights Acesso aberto
dc.title First Description of Natural and Experimental Conjugation between Mycobacteria Mediated by a Linear Plasmid en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Lab Nacl Comp Cient
dc.contributor.institution Univ Autonoma Madrid
dc.contributor.institution Inst Adolfo Lutz Registro
dc.description.affiliation Universidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, Escola Paulista Med, São Paulo, Brazil
dc.description.affiliation Lab Nacl Comp Cient, Petropolis, Brazil
dc.description.affiliation Univ Autonoma Madrid, Fac Med, Dept Prevent Med, Madrid, Spain
dc.description.affiliation Inst Adolfo Lutz Registro, Nucleo TB & Micobacterioses, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, Escola Paulista Med, São Paulo, Brazil
dc.description.sponsorshipID FAPESP: FAPESP - 06/01533-9
dc.identifier.file WOS000301123400109.pdf
dc.identifier.doi 10.1371/journal.pone.0029884
dc.description.source Web of Science
dc.identifier.wos WOS:000301123400109



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