Effect of varicocele on sperm function and semen oxidative stress

Effect of varicocele on sperm function and semen oxidative stress

Author Blumer, Camile Garcia Autor UNIFESP Google Scholar
Restelli, Adriana Ester Autor UNIFESP Google Scholar
Del Giudice, Paula Toni Autor UNIFESP Google Scholar
Soler, Thiesa Butterby Autor UNIFESP Google Scholar
Fraietta, Renato Autor UNIFESP Google Scholar
Nichi, Marcilio Google Scholar
Bertolla, Ricardo Pimenta Autor UNIFESP Google Scholar
Cedenho, Agnaldo Pereira Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract OBJECTIVETo assess the effect of varicocele on sperm DNA integrity, mitochondrial activity, lipid peroxidation and acrosome integrity.PATIENTS and METHODSIn all, 30 patients with a clinically diagnosed varicocele of grade II or III and 32 men without a varicocele were evaluated for sperm DNA fragmentation (comet assay), mitochondrial activity (3,3'-diaminobenzidine assay), lipid peroxidation (malondialdehyde) and acrosome integrity (fluorescent probe labelled peanut agglutinin).RESULTSThe varicocele group showed fewer spermatozoa with intact DNA (grade II, P = 0.040), more cells with inactive mitochondria (class III, P = 0.001), fewer cells with active mitochondria (class I, P = 0.005) and fewer spermatozoa with intact acrosomes (P < 0.001). Finally, no significant differences were observed in lipid peroxidation levels.CONCLUSIONMen with varicocele showed an increase in sperm DNA fragmentation and a reduction in mitochondrial activity and acrosome integrity. However, lipid peroxidation levels remained unchanged.
Keywords varicocele
oxidative stress
DNA damage
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 06/58657-1
Date 2012-01-01
Published in Bju International. Malden: Wiley-Blackwell, v. 109, n. 2, p. 259-265, 2012.
ISSN 1464-4096 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 259-265
Origin http://dx.doi.org/10.1111/j.1464-410X.2011.10240.x
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000299081700025
URI http://repositorio.unifesp.br/handle/11600/34354

Show full item record


File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)




My Account