A novel approach for the characterisation of proteoglycans and biosynthetic enzymes in a snail model

A novel approach for the characterisation of proteoglycans and biosynthetic enzymes in a snail model

Author Gesteira, Tarsis Ferreira Autor UNIFESP Google Scholar
Coulson-Thomas, Vivien Jane Autor UNIFESP Google Scholar
Ogata, Fernando Toshio Autor UNIFESP Google Scholar
Farias, Eduardo Henrique Cunha de Autor UNIFESP Google Scholar
Cavalheiro, Renan Pelluzzi Autor UNIFESP Google Scholar
Lima, Marcelo Andrade Autor UNIFESP Google Scholar
Cunha, Gabriel L. A Autor UNIFESP Google Scholar
Nakayasu, Ernesto S Google Scholar
Almeida, Igor Correia de Google Scholar
Toma, Leny Autor UNIFESP Google Scholar
Nader, Helena Bonciani Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Texas El Paso
Abstract Proteoglycans encompass a heterogeneous group of glycoconjugates where proteins are substituted with linear, highly negatively charged glycosaminoglycan chains. Sulphated glycosaminoglycans are ubiquitous to the animal kingdom of the Eukarya domain. Information on the distribution and characterisation of proteoglycans in invertebrate tissues is limited and restricted to a few species. By the use of multidimensional protein identification technology and immunohistochemistry, this study shows for the first time the presence and tissue localisation of different proteoglycans, such as perlecan, aggrecan, and heparan sulphate proteoglycan, amongst others, in organs of the gastropoda Achatina fulica. Through a proteomic analysis of Golgi proteins and immunohistochemistry of tissue sections, we detected the machinery involved in glycosaminoglycan biosynthesis, related to polymer formation (polymerases), as well as secondary modifications (sulphation and uronic acid epimerization). Therefore, this work not only identifies both the proteoglycan core proteins and glycosaminoglycan biosynthetic enzymes in invertebrates but also provides a novel method for the study of glycosaminoglycan and proteoglycan evolution. (C) 2011 Elsevier B.V. All rights reserved.
Keywords Achatina fulica
Glycosaminoglycan
Acharan sulfate
Immunohistochemistry
Proteomic analysis
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
NIH
Grant number NIH: 2G12RR008124-16A1
NIH: 2G12RR008124-16A1S1
Date 2011-12-01
Published in Biochimica Et Biophysica Acta-proteins and Proteomics. Amsterdam: Elsevier B.V., v. 1814, n. 12, p. 1862-1869, 2011.
ISSN 1570-9639 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 1862-1869
Origin http://dx.doi.org/10.1016/j.bbapap.2011.07.024
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000298363200031
URI http://repositorio.unifesp.br/handle/11600/34240

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