Induction of proinflammatory cytokines and nitric oxide by Trypanosoma cruzi in renal cells

Induction of proinflammatory cytokines and nitric oxide by Trypanosoma cruzi in renal cells

Author Oliveira, Gabriel M. de Google Scholar
Yoshida, Nobuko Google Scholar
Higa, Elisa M. S. Autor UNIFESP Google Scholar
Shenkman, Sergio Google Scholar
Alves, Monique Google Scholar
Staquicini, Daniela Google Scholar
Cascabulho, Cynthia Google Scholar
Schor, Nestor Autor UNIFESP Google Scholar
Institution Fundacao Oswaldo Cruz
Dept Microbiol Imunol & Parasitol
Universidade Federal de São Paulo (UNIFESP)
Fiocruz MS
Abstract Chagas disease is typically associated with cardiac involvement. During the acute phase of murine infection with Trypanosoma cruzi, severe acute myocarditis can develop. Prior to cardiac alteration, however, infected mice present with renal inflammatory infiltration causing acute kidney injury due to an ischemia/reperfusion lesion. Thus, the present study was undertaken in order to evaluate whether the parasites or some of their components would directly affect renal cells. As such, this study employed kidney cell lines (mesangial, epithelial, and proximal tubular) that mimic different regions of the renal system. Mesangial cells are more resistant to infection, showing reduced parasite internalization relative to epithelial and proximal tubular cells. Upon infection, mesangial cells produced more nitric oxide, tumor factor necrosis-alpha, and interferon-gamma and showed decreased viability when compared to the other cell lines. These results indicate that the resistance of mesangial cells to infection may be related to the increased expression of nitric oxide and proinflammatory cytokines. Conversely, the high levels of nitric oxide produced by these cells caused impairment of cell integrity and viability. Higher nitric oxide concentrations promote cellular injury and can be involved in the genesis of ischemia/reperfusion lesions in acute kidney injury.
Language English
Date 2011-08-01
Published in Parasitology Research. New York: Springer, v. 109, n. 2, p. 483-491, 2011.
ISSN 0932-0113 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 483-491
Origin http://dx.doi.org/10.1007/s00436-011-2279-6
Access rights Closed access
Type Article
Web of Science ID WOS:000292932100028
URI http://repositorio.unifesp.br/handle/11600/33914

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