Insights on PRAME and osteosarcoma by means of gene expression profiling

Insights on PRAME and osteosarcoma by means of gene expression profiling

Author Toledo, Silvia Regina Caminada de Autor UNIFESP Google Scholar
Zago, Marco Antonio Google Scholar
Oliveira, Indhira Dias Autor UNIFESP Google Scholar
Proto-Siqueira, Rodrigo Google Scholar
Okamoto, Oswaldo K. Google Scholar
Severino, Patricia Google Scholar
Vencio, Ricardo Z. N. Google Scholar
Gamba, Francine Tesser Autor UNIFESP Google Scholar
Silva, Wilson A. Google Scholar
Moreira-Filho, Carlos A. Google Scholar
Dalla Torre, Cristiane Arruda Autor UNIFESP Google Scholar
Seixas Alves, Maria Tereza Autor UNIFESP Google Scholar
Garcia-Filho, Reynaldo J. Autor UNIFESP Google Scholar
Simpson, Andrew J. G. Google Scholar
Petrilli, Antonio Sergio Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Albert Einstein Res & Educ Inst
Ludwig Inst
Abstract Osteosarcoma (OS) is the most frequent bone tumor in children and adolescents. Tumor antigens are encoded by genes that are expressed in many types of solid tumors but are silent in normal tissues, with the exception of placenta and male germ-line cells. It has been proposed that antigen tumors are potential tumor markers.The premise of this study is that the identification of novel OS-associated transcripts will lead to a better understanding of the events involved in OS pathogenesis and biology.We analyzed the expression of a panel of seven tumor antigens in OS samples to identify possible tumor markers. After selecting the tumor antigen expressed in most samples of the panel, gene expression profiling was used to identify osteosarcoma-associated molecular alterations. A microarray was employed because of its ability to accurately produce comprehensive expression profiles.PRAME was identified as the tumor antigen expressed in most OS samples; it was detected in 68% of the cases. Microarray results showed differences in expression for genes functioning in cell signaling and adhesion as well as extracellular matrix-related genes, implying that such tumors could indeed differ in regard to distinct patterns of tumorigenesis.The hypothesis inferred in this study was gathered mostly from available data concerning other kinds of tumors. There is circumstantial evidence that PRAME expression might be related to distinct patterns of tumorigenesis. Further investigation is needed to validate the differential expression of genes belonging to tumorigenesis-related pathways in PRAME-positive and PRAME-negative tumors.
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
GRAACC (Grupo de Apoio ao Adolescente e Crianca com Cancer)
Grant number FAPESP: 04/12150-8
FAPESP: 07/53869-3
Date 2011-07-01
Published in Journal of Orthopaedic Science. Tokyo: Springer Tokyo, v. 16, n. 4, p. 458-466, 2011.
ISSN 0949-2658 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 458-466
Access rights Closed access
Type Article
Web of Science ID WOS:000293001600019

Show full item record


File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)




My Account