Prevalence of virulence factors in Escherichia coli isolated from healthy animals and water sources in Brazil

Prevalence of virulence factors in Escherichia coli isolated from healthy animals and water sources in Brazil

Author Carlos, Camila Google Scholar
Alexandrino, Fabiana Google Scholar
Vieira, Monica A. M. Autor UNIFESP Google Scholar
Stoppe, Nancy C. Google Scholar
Sato, Maria Ines Z. Google Scholar
Gomes, Tania A. T. Autor UNIFESP Google Scholar
Ottoboni, Laura M. M. Google Scholar
Institution Universidade Estadual de Campinas (UNICAMP)
Universidade Federal de São Paulo (UNIFESP)
Co Ambiental Estado São Paulo CETESB
Abstract The aim of this work was to verify the presence of seven virulence factors (ST. LT, eae, stx(1), stx(2), INV and EAEC) among Escherichia coli strains isolated from healthy humans, bovines, chickens, sheep, pigs and goats, from two sewage treatment plants and from the Tiete River. We have found a high prevalence of eae, stx(1) and stx(2) in ruminants. the EAEC gene was only found in humans and sewage. No strains presented ST, LT or INV. BOX-PCR fingerprints revealed a high diversity among the strains analysed and a non-clonal origin of strains that presented the same virulence factors. Therefore, we concluded that ruminants may constitute an important reservoir of most diarrheagenic E. coli in Brazil, except for EAEC strains. These results emphasize the importance of the identification of the animal source of fecal contamination for the correct water risk assessment.
Keywords BOX-PCR
Escherichia coli
fecal contamination
virulence factors
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: FAPESP 2007/55312-6
FAPESP: FAPESP 2007/57025-4
Date 2011-03-01
Published in Journal of Water and Health. London: Iwa Publishing, v. 9, n. 1, p. 138-142, 2011.
ISSN 1477-8920 (Sherpa/Romeo, impact factor)
Publisher Iwa Publishing
Extent 138-142
Access rights Closed access
Type Article
Web of Science ID WOS:000289387900013

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