B-1 cells temper endotoxemic inflammatory responses

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dc.contributor.author Barbeiro, Denise Frediani
dc.contributor.author Barbeiro, Hermes Vieira
dc.contributor.author Faintuch, Joel
dc.contributor.author Kubo Ariga, Suely K.
dc.contributor.author Mariano, Mario [UNIFESP]
dc.contributor.author Popi, Ana Flavia [UNIFESP]
dc.contributor.author Souza, Heraldo Possolo de
dc.contributor.author Velasco, Irineu Tadeu
dc.contributor.author Soriano, Francisco Garcia
dc.date.accessioned 2016-01-24T14:06:16Z
dc.date.available 2016-01-24T14:06:16Z
dc.date.issued 2011-03-01
dc.identifier http://dx.doi.org/10.1016/j.imbio.2010.08.002
dc.identifier.citation Immunobiology. Jena: Elsevier Gmbh, Urban & Fischer Verlag, v. 216, n. 3, p. 302-308, 2011.
dc.identifier.issn 0171-2985
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/33526
dc.description.abstract Sepsis syndrome is caused by inappropriate immune activation due to bacteria and bacterial components released during infection. This syndrome is the leading cause of death in intensive care units. Specialized B-lymphocytes located in the peritoneal and pleural cavities are known as B-1 cells. These cells produce IgM and IL-10, both of which are potent regulators of cell-mediated immunity. It has been suggested that B-1 cells modulate the systemic inflammatory response in sepsis. in this study, we conducted in vitro and in vivo experiments in order to investigate a putative role of B-1 cells in a murine model of LPS-induced sepsis. Macrophages and B-1 cells were studied in monocultures and in co-cultures. the B-1 cells produced the anti-inflammatory cytokine IL-10 in response to LPS. in the B-1 cell-macrophage co-cultures, production of proinflammatory mediators (TNF-alpha, IL-6 and nitrite) was lower than in the macrophage monocultures, whereas that of IL-10 was higher in the co-cultures. Co-culture of B-1 IL-10(-/-) cells and macrophages did not reduce the production of the proinflammatory mediators (TNF-alpha, IL-6 and nitrite). After LPS injection, the mortality rate was higher among Balb/Xid mice, which are B-1 cell deficient, than among wild-type mice (65.0% vs. 0.0%). the Balb/Xid mice also presented a proinflammatory profile of TNF-alpha, IL-6 and nitrite, as well as lower levels of IL-10. in the early phase of LPS stimulation, B-1 cells modulate the macrophage inflammatory response, and the main molecular pathway of that modulation is based on IL-10-mediated intracellular signaling. (C) 2010 Elsevier GmbH. All rights reserved. en
dc.format.extent 302-308
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Immunobiology
dc.rights Acesso restrito
dc.subject Cytokines en
dc.subject Inflammation en
dc.subject LPS en
dc.subject Macrophage en
dc.subject Sepsis en
dc.title B-1 cells temper endotoxemic inflammatory responses en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ São Paulo, Fac Med, Sch Med, Clin Lab Emergency Med, BR-01246903 São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Sch Med, Hosp Clin, Dept Gastroenterol, BR-01246903 São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.identifier.doi 10.1016/j.imbio.2010.08.002
dc.description.source Web of Science
dc.identifier.wos WOS:000288722500004


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