Genotoxic effects of (-)-cubebin in somatic cells of mice

Genotoxic effects of (-)-cubebin in somatic cells of mice

Author Maistro, Edson Luis Google Scholar
Marcondes Natel, Andre Vinicius Google Scholar
Bianco de Souza, Gustavo Henrique Google Scholar
Perazzo, Fabio Ferreira Autor UNIFESP Google Scholar
Institution Univ Estadual Paulista
Univ Fed Ouro Preto
Universidade Federal de São Paulo (UNIFESP)
Abstract (-)-Cubebin belongs to the dibenzylbutyrolactone lignan group, which is widely distributed in the plant kingdom. Because this compound shows interesting biological activities, it is extremely important to evaluate its possible genotoxic activity to allow its safe use in humans. Thus, the present study was performed to investigate the genotoxicity potential activity of (-)-cubebin assessed by two assays: micronucleus in bone marrow cells and comet test in peripheral blood leukocytes of Swiss mice. in the (-)-cubebin dose range-finding assays, the maximum tolerated dose was greater than 2000 mg kg(-1). the compound was administered by an oral route at single doses of 250, 500 and 2000 mg kg(-1) body weight. Cytotoxicity was assessed by scoring 200 consecutive total polychromatic (PCE) and normochromatic (NCE) erythrocytes (PCE/NCE ratio). Under our experimental conditions, micronucleus and comet assays, respectively, showed that (-)-cubebin caused dose-related clastogenic and genotoxic effects in the somatic cells investigated. PCE/NCE ratio showed no cytotoxicity for the three doses of the compound. the data suggest caution in the ingestion of (-)-cubebin by humans, especially at high doses. Copyright (C) 2010 John Wiley & Sons, Ltd.
Keywords (-)-cubebin
micronucleus test
comet assay
clastogenicity
Piper cubeba
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number CNPq: 306544/2006-7
FAPESP: 2008/51175-7
Date 2011-03-01
Published in Journal of Applied Toxicology. Malden: Wiley-Blackwell, v. 31, n. 2, p. 185-189, 2011.
ISSN 0260-437X (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 185-189
Origin http://dx.doi.org/10.1002/jat.1616
Access rights Closed access
Type Article
Web of Science ID WOS:000288473100012
URI http://repositorio.unifesp.br/handle/11600/33503

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