Comparative study of liponucleosides in Langmuir monolayers as cell membrane models

Comparative study of liponucleosides in Langmuir monolayers as cell membrane models

Author Montanha, E. A. Google Scholar
Caseli, L. Autor UNIFESP Google Scholar
Kaczmarek, O. Google Scholar
Liebscher, J. Google Scholar
Huster, D. Google Scholar
Oliveira, O. N. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Humboldt Univ
Univ Leipzig
Abstract Liponucleosides may assist the anchoring of nucleic acid nitrogen bases into biological membranes for tailored nanobiotechnological applications. To this end precise knowledge about the biophysical and chemical details at the membrane surface is required. in this paper, we used Langmuir monolayers as simplified cell membrane models and studied the insertion of five lipidated nucleosides. These molecules varied in the type of the covalently attached lipid group, the nucleobase, and the number of hydrophobic moieties attached to the nucleoside. All five lipidated nucleosides were found to be surface-active and capable of forming stable monolayers. They could also be incorporated into dipalmitoylphosphatidylcholine (DPPC) monolayers, four of which induced expansion in the surface pressure isotherm and a decrease in the surface compression modulus of DPPC. in contrast, one nucleoside possessing three alkyl chain modifications formed very condensed monolayers and induced film condensation and an increase in the compression modulus for the DPPC monolayer, thus reflecting the importance of the ability of the nucleoside molecules to be arranged in a closely packed manner. the implications of these results lie on the possibility of tuning nucleic acid pairing by modifying structural characteristics of the liponucleosides. (C) 2010 Elsevier B.V. All rights reserved.
Keywords Langmuir monolayers
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
nBioNet (Brazil)
Federal Ministry of Education and Research
Grant number Federal Ministry of Education and Research: BMBF 0312027C
Federal Ministry of Education and Research: 0312027D
Federal Ministry of Education and Research: 0312018
Date 2011-01-01
Published in Biophysical Chemistry. Amsterdam: Elsevier B.V., v. 153, n. 2-3, p. 154-158, 2011.
ISSN 0301-4622 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 154-158
Access rights Closed access
Type Article
Web of Science ID WOS:000287294000003

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