Oral tolerance reduces Th17 cells as well as the overall inflammation in the central nervous system of EAE mice

Oral tolerance reduces Th17 cells as well as the overall inflammation in the central nervous system of EAE mice

Author Peron, Jean Pierre S. Google Scholar
Yang, Kayong Google Scholar
Chen, Mei-Ling Google Scholar
Brandao, Wesley Nogueira Google Scholar
Basso, Alexandre S. Autor UNIFESP Google Scholar
Commodaro, Alessandra G. Google Scholar
Weiner, Howard L. Google Scholar
Rizzo, Luiz V. Google Scholar
Institution Universidade de São Paulo (USP)
Harvard Univ
Universidade Federal de São Paulo (UNIFESP)
Albert Einstein Jewish Inst Educ & Res
Abstract Multiple sclerosis (MS) is an autoimmune disease characterized by inflammatory immune response directed against myelin antigens of the central nervous system. in its murine model, EAE, Th17 cells play an important role in disease pathogenesis. These cells can induce blood-brain barrier disruption and CNS immune cells activation, due to the capacity to secrete high levels of IL-17 and IL-22 in an IL-6 + TGF-beta dependent manner. Thus, using the oral tolerance model, by which 200 mu g of MOG 35-55 is given orally to C57BL/6 mice prior to immunization, we showed that the percentage of Th17 cells as well as IL-17 secretion is reduced both in the periphery and also in the CNS of orally tolerated animals. Altogether, our data corroborates with the pathogenic role of IL-17 and IFN-gamma in EAE, as its reduction after oral tolerance, leads to an overall reduction of pro-inflammatory cytokines, such as IL-1 alpha, IL-6, IL-9, IL-12p70 and the chemokines MIP-1 beta, RANTES, Eotaxin and KC in the CNS. It is noteworthy that this was associated to an increase in IL-10 levels. Thus, our data clearly show that disease suppression after oral tolerance induction, correlates with reduction in target organ inflammation, that may be caused by a reduced Th1/Th17 response. Crown Copyright (c) 2010 Published by Elsevier B.V. All rights reserved.
Keywords EAE
Th17
CNS inflammation
Oral tolerance
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number CAPES: 4499-05-0
CAPES: PNPD 0188085
FAPESP: 2009/13109-5
Date 2010-10-08
Published in Journal of Neuroimmunology. Amsterdam: Elsevier B.V., v. 227, n. 1-2, p. 10-17, 2010.
ISSN 0165-5728 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 10-17
Origin http://dx.doi.org/10.1016/j.jneuroim.2010.06.002
Access rights Closed access
Type Article
Web of Science ID WOS:000283007500002
URI http://repositorio.unifesp.br/handle/11600/32986

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