Properties of lipophilic nucleoside monolayers at the air-water interface

Properties of lipophilic nucleoside monolayers at the air-water interface

Author Montanha, E. A. Google Scholar
Pavinatto, F. J. Google Scholar
Caseli, L. Autor UNIFESP Google Scholar
Kaczmarek, O. Google Scholar
Liebscher, J. Google Scholar
Huster, D. Google Scholar
Oliveira, O. N. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Humboldt Univ
Univ Leipzig
Abstract The capability of self-assembly and molecular recognition of biomolecules is essential for many nanotechnological applications, as in the use of alkyl-modified nucleosides and oligonucleotides to increase the cellular uptake of DNA and RNA. in this study, we show that a lipophilic nucleoside, which is an isomer mixture of 2'-palmitoyluridin und 3'-palmitoyluridin, forms Langmuir monolayers and Langmuir-Blodgett films as a typical amphiphile, though with a smaller elasticity. the nucleoside may be incorporated into dipalmitoyl phosphatidyl choline (DPPC) monolayers that serve as a simplified cell membrane model. the molecular-level interactions between the nucleoside and DPPC led to a remarkable condensation of the mixed monolayer, which affected both surface pressure and surface potential isotherms. the morphology of the mixed monolayers was dominated by the small domains of the nucleoside. the mixed monolayers could be deposited onto solid substrates as a one-layer Langmuir Blodgett film that displayed UV-vis absorption spectra typical of aggregated nucleosides owing to the interaction between the nucleoside and DPPC. the formation of solid films with DNA building blocks in the polar heads may open the way for devices and sensors be produced to exploit their molecular recognition properties. (C) 2010 Elsevier B.V. All rights reserved.
Keywords Lipophilic nucleosides
Langmuir monolayers
Langmuir-Blodgett films
Model membrane
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Federal Ministry of Education and Research (BMBF)
Grant number Federal Ministry of Education and Research (BMBF): 0312027 C
Federal Ministry of Education and Research (BMBF): D 0312018
Date 2010-06-01
Published in Colloids and Surfaces B-biointerfaces. Amsterdam: Elsevier B.V., v. 77, n. 2, p. 161-165, 2010.
ISSN 0927-7765 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 161-165
Access rights Closed access
Type Article
Web of Science ID WOS:000276753600006

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