Enhancement on the Europium emission band of Europium chlortetracycline complex in the presence of LDL

Enhancement on the Europium emission band of Europium chlortetracycline complex in the presence of LDL

Author Teixeira, Luciane dos Santos Google Scholar
Grasso, Andrea Nastri Google Scholar
Monteiro, Andrea Moreira Google Scholar
Figueiredo Neto, A. M. Google Scholar
Vieira, Nilson Dias Google Scholar
Gidlund, Magnus Google Scholar
Courrol, Lilia Coronato Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
IPEN CNEN
Universidade de São Paulo (USP)
Abstract Low-density lipoprotein (LDL) particles are the major cholesterol-carrying lipoprotein in the human circulation from the liver to peripheral tissues. High levels of LDL-Cholesterol (LDL-C) are known risk factor for the development of coronary artery disease (CAD). the most common approach to determine the LDLC in the clinical laboratory involves the Friedewald formula. However, in certain situations, this approach is inadequate. in this paper we report on the enhancement on the Europium emission band of Europium chlortetracycline complex (CTEu) in the presence of LDL. the emission intensity at 615 nm of the CTEu increases with increasing amounts of LDL. This phenomenon allowed us to propose a method to determine the LDL concentration in a sample composed by an aqueous solution of LDL. With this result we obtained LDL calibration curve, LOD (limit of detection) of 0.49 mg/mL and SD (standard deviation) of 0.003. We observed that CTEu complex provides a wider dynamic concentration-range for LDL determination than that from Eu-tetracycline previously. the averaged emission lifetimes of the CTEu and CTEu with LDL (1.5 mg/mL) complexes were measured as 15 and 46 Its, respectively. Study with some metallic interferents is presented. (C) 2010 Elsevier Inc. All rights reserved.
Keywords LDL
Europium
Fluorescence
Biosensor
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
National institute of Science and Technology of Complex Fluids (INCT-FCx)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2010-05-01
Published in Analytical Biochemistry. San Diego: Academic Press Inc Elsevier Science, v. 400, n. 1, p. 19-24, 2010.
ISSN 0003-2697 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 19-24
Origin http://dx.doi.org/10.1016/j.ab.2010.01.030
Access rights Closed access
Type Article
Web of Science ID WOS:000275840400003
URI http://repositorio.unifesp.br/handle/11600/32506

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