The kinin B-2 receptor gene structure, product processing and expression in adult and fetal rats: evidence for gene evolution

The kinin B-2 receptor gene structure, product processing and expression in adult and fetal rats: evidence for gene evolution

Author Franca, Camila Estevao de Autor UNIFESP Google Scholar
Vicari, Camilo Alfredo Faigle Autor UNIFESP Google Scholar
Piza, Ailton Marques Autor UNIFESP Google Scholar
Geroldo, Elaine Aparecida Autor UNIFESP Google Scholar
Beçak, Maria Luiza Google Scholar
Becak, Willy Google Scholar
Stocco, Rita de Cassia Google Scholar
Lindsey, Charles Julian Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Inst Butantan
Abstract We examined the structure of the rat kinin B-2 receptor gene (KB(2)r) and encoding messenger RNA (mRNA) processing. Differently from the closely related mouse and rabbit genes that have three exons and two introns, the rat gene purportedly consists of four exons and three introns. There are two purported gene products; one of them contains an upstream similar to 180-bp open reading frame region (exon-X) potentially expressed as a result of alternative processing. To examine the processing of rat KB(2)r mRNA, cDNA amplicons were generated using primer pairs directed towards 5' or 3' exon or intron flanking regions. Analyses of intron/exon primary cDNA amplicons showed that introns 1 to 3 are removed sequentially and that exon-X removal follows that of intron-3. No evidence was found for exon-X expression in polyadenylated (mature) mRNA of adult Wistar, Wistar Kyoto, spontaneously hypertensive or Sprague-Dawley rat tissues. Nor was exon-X detected in tissues subject to inflammatory stimulus expressing B-1 kinin receptor mRNA or in 1- to 21-day-old rat embryos or fetuses. the lack of evidence for the expression of exon-X in mature mRNA indicates that the structure of the rat gene is similar to that of the mouse, rabbit and human genes, all consisting of three exons and two introns. the exon-X fragment may result from interstitial gene duplication, be a fragment of the ancestral gene, or most likely heterologous transposon insertion of an exon-like fragment into intron-2 of the KB(2)r gene.
Keywords Alternative processing
Rat embryos
Kinin B-2 receptor gene
Language English
Date 2010-01-01
Published in Genetics and Molecular Research. Ribeirao Preto: Funpec-editora, v. 9, n. 1, p. 215-230, 2010.
ISSN 1676-5680 (Sherpa/Romeo, impact factor)
Publisher Funpec-editora
Extent 215-230
Origin http://dx.doi.org/10.4238/vol9-1gmr681
Access rights Open access Open Access
Type Review
Web of Science ID WOS:000277326200016
URI http://repositorio.unifesp.br/handle/11600/32056

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