Author |
Santos, Ronaldo Vagner Thomatieli dos
![]() ![]() Caperuto, Erico C. ![]() Mello, Marco Tulio de ![]() ![]() Batista, Miguel L. ![]() Rosa, Luis F. B. P. C. ![]() |
Institution | Universidade Federal de São Paulo (UNIFESP) Universidade de São Paulo (USP) |
Abstract | P>Reductions in plasma glutamine are observed after prolonged exercise. Three hypotheses can explain such a decrease: (i) high demand by the liver and kidney; (ii) impaired release from muscles; and (iii) decreased synthesis in skeletal muscle. the present study investigated the effects of exercise on glutamine synthesis and transport in rat skeletal muscle.Rats were divided into three groups: (i) sedentary (SED; n = 12); (ii) rats killed 1 h after the last exercise bout (EX-1; n = 15); and (iii) rats killed 24 h after the last exercise bout (EX-24; n = 15). Rats in the trained groups swam 1 h/day, 5 days/week for 6 weeks with a load equivalent to 5.5% of their bodyweight.Plasma glutamine and insulin were lower and corticosterone was higher in EX-1 compared with SED rats (P < 0.05 and P < 0.01, respectively). Twenty-four hours after exercise (EX-24), plasma glutamine was restored to levels seen in SED rats, whereas insulin levels were higher (P < 0.001) and costicosterone levels were lower (P < 0.01) than in EX-1. in the soleus, ammonia levels were lower in EX-1 than in SED rats (P < 0.001). After 24 h, glutamine, glutamate and ammonia levels were lower in EX-24 than in SED and EX-1 rats (P < 0.001). Soleus glutamine synthetase (GS) activity was increased in EX-1 and was decreased in EX-24 compared with SED rats (both P < 0.001).The decrease in plasma glutamine concentration in EX-1 is not mediated by GS or glutamine transport in skeletal muscle. However, 24 h after exercise, lower GS may contribute to the decrease in glutamine concentration in muscle. |
Keywords |
Aerobic exercise
Glutamine synthetase Glutamine transport Moderate exercise Soleus |
Language | English |
Sponsor | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) |
Grant number |
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Date | 2009-08-01 |
Published in | Clinical and Experimental Pharmacology and Physiology. Malden: Wiley-Blackwell Publishing, Inc, v. 36, n. 8, p. 770-775, 2009. |
ISSN | 0305-1870 (Sherpa/Romeo, impact factor) |
Publisher | Wiley-Blackwell |
Extent | 770-775 |
Origin |
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Access rights | Closed access |
Type | Article |
Web of Science ID | WOS:000268458200009 |
URI | https://repositorio.unifesp.br/handle/11600/31734 |
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