Glycosaminoglycan chains from alpha(5)beta(1) integrin are involved in fibronectin-dependent cell migration

Glycosaminoglycan chains from alpha(5)beta(1) integrin are involved in fibronectin-dependent cell migration

Author Franco, Celia R. C. Autor UNIFESP Google Scholar
Trindade, Edvaldo S. Autor UNIFESP Google Scholar
Rocha, Hugo A. O. Autor UNIFESP Google Scholar
Silveira, Rafael Bertoni da Autor UNIFESP Google Scholar
Paludo, Katia Sabrina Google Scholar
Chammas, Roger Google Scholar
Veiga, Silvio S. Google Scholar
Nader, Helena B. Autor UNIFESP Google Scholar
Dietrich, Carl P. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Fed Parana
Univ Fed Rio Grande do Norte
Universidade de São Paulo (USP)
Abstract alpha(5)beta(1) integrin from both wild-type CHO cells (CHO-K1) and deficient in proteoglycan biosynthesis (CHO-745) is post-translationally modified by glycosaminoglycan chains. We demonstrated this using [S-35]sulfate metabolic labeling of the cells, enzymatic degradation, immunoprecipitation reaction with monoclonal antibody, fluorescence microscopy, and flow cytometry. the alpha(5)beta(1) integrin heterodimer is a hybrid proteoglycan containing both chondroitin and heparan sulfate chains. Xyloside inhibition of sulfate incorporation into alpha(5)beta(1) integrin also supports that integrin is a proteoglycan. Also. cells grown with xyloside adhered on fibronectin with no alteration in alpha(5)beta(1) integrin expression. However, haptotactic motility on fibronectin declined in cells grown with xyloside or chlorate as compared with controls. Thus, alpha(5)beta(1) integrin is a proteoglycan and the glycosaminoglycan chains of the integrin influence cell motility on fibronectin. Similar glycosylation of alpha(5)beta(1) integrin was observed in other normal and malignant cells, suggesting that this modification is conserved and important in the function of this integrin. Therefore, these glycosaminoglycan chains of alpha(5)beta(1) integrin are involved in cellular migration on fibronectin.
Keywords integrin
glycosaminoglycan
migration on fibronectin
adhesion on fibronectin
proteoglycan
Language English
Date 2009-08-01
Published in Biochemistry and Cell Biology-biochimie Et Biologie Cellulaire. Ottawa: Canadian Science Publishing, Nrc Research Press, v. 87, n. 4, p. 677-686, 2009.
ISSN 0829-8211 (Sherpa/Romeo, impact factor)
Publisher Canadian Science Publishing, Nrc Research Press
Extent 677-686
Origin http://dx.doi.org/10.1139/O09-047
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000269762100012
URI http://repositorio.unifesp.br/handle/11600/31697

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