A role for regulatory T cells in renal acute kidney injury

A role for regulatory T cells in renal acute kidney injury

Author Monteiro, Rebecca M. M. Autor UNIFESP Google Scholar
Camara, Niels O. S. Autor UNIFESP Google Scholar
Rodrigues, Mauricio M. Autor UNIFESP Google Scholar
Tzelepis, Fanny Autor UNIFESP Google Scholar
Damiao, Marcio J. Autor UNIFESP Google Scholar
Cenedeze, Marcos A. Autor UNIFESP Google Scholar
Teixeira, Vicente de Paula A. Google Scholar
Reis, Marlene A. dos Google Scholar
Pacheco-Silva, Alvaro Autor UNIFESP Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Hosp Albert Einstein
Abstract Ischemia reperfusion injury (IRI) is a potential contributor for the development of chronic allograft nephropathy. T cells are important mediators of injury, even in the absence of alloantigens. We performed a depletion of TCD4(+)CTLA4(+)Foxp3(+) cells with anti-CD25(PC61), a treatment with anti-GITR (DTA-1) and rat-IgG, followed by 45 min of ischemia and 24/72 h of reperfusion, and then analyzed blood urea, kidney histopathology and gene expression in kidneys by QReal Time PCR. After 24 h of reperfusion, depletion of TCD4(+)CTLA4(+)Foxp3(+) cells reached 30.3%(spleen) and 67.8%(lymph nodes). 72 h after reperfusion depletion reached 43.1%(spleen) and 90.22%(lymph nodes) and depleted animals presented with significantly poorer renal function, while DTA-1 (anti-GITR)-treated ones showed a significant protection, all compared to serum urea from control group (IgG: 150.10 +/- 50.04; PC61: 187.23 +/- 31.38; DTA-1: 64.53 +/- 25.65, mg/dL, p<0.05). These data were corroborated by histopathology. We observed an increase of HO-1 expression in animals treated with DTA-1 at 72 h of reperfusion with significant differences. Thus, our results suggest that PC61 (anti-CD25) mAb treatment is deleterious, while DTA-1 (anti-GITR) mAb treatment presents a protective role in the renal IRI, indicating that some regulatory populations of T cells might have a role in IRI. (C) 2009 Elsevier B.V. All rights reserved.
Keywords Acute renal failure
Ischemia and reperfusion injury
TCD4(+) lymphocytes
Anti-CD25 (PC61)
Anti-GITR (DTA-1)
Language English
Date 2009-05-01
Published in Transplant Immunology. Amsterdam: Elsevier B.V., v. 21, n. 1, p. 50-55, 2009.
ISSN 0966-3274 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 50-55
Origin http://dx.doi.org/10.1016/j.trim.2009.02.003
Access rights Closed access
Type Article
Web of Science ID WOS:000265894600009
URI http://repositorio.unifesp.br/handle/11600/31505

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