Endurance training induces depot-specific changes in IL-10/TNF-alpha ratio in rat adipose tissue

Endurance training induces depot-specific changes in IL-10/TNF-alpha ratio in rat adipose tissue

Author Lira, F. S. Google Scholar
Rosa, J. C. Autor UNIFESP Google Scholar
Yamashita, A. S. Google Scholar
Koyama, C. H. Google Scholar
Batista, M. L. Google Scholar
Seelaender, M. Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Univ Mogi das Cruzes
Abstract White adipose tissue (WAT) is the source of pro- and anti-inflammatory cytokines and recently, it has been recognized as an important source of interleukin 10 (IL-10). Acute physical exercise is known to induce an anti-inflammatory cytokine profile, however, the effect of chronic physical exercise on the production of IL-10 by WAT has never been examined. We assessed IL-10 and TNF-alpha concentration in WAT of rats engaged in endurance training. Animals were randomly assigned to either a sedentary control group (S, n = 7) or an endurance trained group (T, n = 8). Trained rats ran on a treadmill 5 days/wk for 8 wk (55-65% VO(2max). Detection of IL-10 and TNF-alpha protein and mRNA expression, as well as the gene expression of PPAR-gamma, and immunocytochemistry to detect mononuclear phagocytes were carried out. A reduction in absolute retroperitoneal adipose tissue (RPAT) weight in T (44%; p < 0.01), when compared with S was observed. IL-10 concentration was increased (1.5-fold, p < 0.05), to a higher extent than that of TNF-alpha (66%. p < 0.05) in the mesenteric adipose tissue (MEAT) of the trained group, while no change related to training was observed in RPAT. in MEAT, IL-10/TNF-alpha ratio was increased in T, when compared with S (30%; p < 0.05). PPAR-gamma gene expression was increased in T (1.1-fold; p < 0.01), when compared with S in the same adipose depot. No monocyte infiltration was found. in conclusion, exercise training induced increased IL-10 expression in the mesenteric depot, resulting in a modified IL-10/TNF-alpha ratio. We also conclude that WAT presents a depot-specific response to endurance training regarding the studied aspects. (C) 2008 Elsevier B.V. All rights reserved.
Keywords Endurance training
Adipose tissue
IL-10
TNF-alpha
PPAR-gamma
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 04111588-0
FAPESP: 05101445-0
Date 2009-02-01
Published in Cytokine. London: Academic Press Ltd Elsevier B.V., v. 45, n. 2, p. 80-85, 2009.
ISSN 1043-4666 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 80-85
Origin http://dx.doi.org/10.1016/j.cyto.2008.10.018
Access rights Closed access
Type Article
Web of Science ID WOS:000264038900005
URI http://repositorio.unifesp.br/handle/11600/31292

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