Genotoxicity and cytotoxicity in multiple organs induced by titanium miniplates in Wistar rats

Genotoxicity and cytotoxicity in multiple organs induced by titanium miniplates in Wistar rats

Author Piozzi, Rodrigo Google Scholar
Ribeiro, Daniel Araki Autor UNIFESP Google Scholar
Marques Padovan, Luis Eduardo Google Scholar
Filho, Hugo Nary Google Scholar
Matsumoto, Mariza Akemi Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract Internal fixture materials are currently used as metallic biomaterials in rehabilitation of human body. Nevertheless, metal release clue to corrosion phenomena appears to play a crucial role for human health. Thus, the goal of the present study was to evaluate whether liver, kidney, and lung are particularly sensitive organs for DNA damaging and cytotoxicity following implantation of internal fixture materials composed by titanium alloy in vivo. A total of 18 rats underwent surgical titanium miniplates in their tibias, being randomly distributed into three groups: 30 days, 90 days, and 190 (lays after implantation. A total of six animals served as negative control (animals that not received any implant). After experimental design, the lung, liver, and kidney were removed for histopatological and genotoxic analysis as depicted by H.E. stain and single cell gel (comet) assay, respectively. No significant statistically differences (p > 0.05) for DNA damaging were found to all experimental groups when compared to negative control for all organs evaluated. in addition, no remarkable morphological alterations were detected under histopathological analysis. Taken together, Such results suggest that titanium miniplates are neither able to induce DNA damage in multiple organs nor to cause some abnormalities in lung, liver, and kidney. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 88A: 342-347, 2009
Keywords titanium
genotoxicity
cytoxicity
rat
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Date 2009-02-01
Published in Journal of Biomedical Materials Research Part A. Hoboken: Wiley-liss, v. 88A, n. 2, p. 342-347, 2009.
ISSN 1549-3296 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 342-347
Origin http://dx.doi.org/10.1002/jbm.a.31876
Access rights Closed access
Type Article
Web of Science ID WOS:000262228300008
URI http://repositorio.unifesp.br/handle/11600/31276

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