GCN2 activation and eIF2 alpha phosphorylation in the maturation of mouse oocytes

GCN2 activation and eIF2 alpha phosphorylation in the maturation of mouse oocytes

Author Alves, Viviane S. Autor UNIFESP Google Scholar
Motta, Fabiana L. Autor UNIFESP Google Scholar
Roffe, Martin Autor UNIFESP Google Scholar
Delamano, Arthur Autor UNIFESP Google Scholar
Pesquero, Joao B. Autor UNIFESP Google Scholar
Castilho, Beatriz A. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract GCN2 is one of the four mammalian kinases that phosphorylate the alpha subunit of the translation initiation factor 2 (eIF2 alpha) in a variety of stress situations, resulting in protein synthesis inhibition. GCN2 is involved in regulating metabolism, feeding behavior and memory in rodents. We show here that, relative to other cells, the beta isoform of the GCN2 transcript and the GCN2 protein are highly abundant in unfertilized mouse eggs. in addition, GCN2 in these cells is active, resulting in elevated levels of phosphorylated eIF2 alpha. After fertilization, eIF2 alpha phosphorylation decreases drastically. These results suggest that GCN2 mediated translational control may contribute to regulatory mechanisms operating during oocyte maturation. (C) 2008 Elsevier Inc. All rights reserved.
Keywords Oocyte
Protein synthesis
Translational control
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Date 2009-01-02
Published in Biochemical and Biophysical Research Communications. San Diego: Academic Press Inc Elsevier Science, v. 378, n. 1, p. 41-44, 2009.
ISSN 0006-291X (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 41-44
Origin http://dx.doi.org/10.1016/j.bbrc.2008.10.161
Access rights Closed access
Type Article
Web of Science ID WOS:000261779800009
URI http://repositorio.unifesp.br/handle/11600/31244

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