Heterogeneity of proteins expressed by Brazilian Sporothrix schenckii isolates

Heterogeneity of proteins expressed by Brazilian Sporothrix schenckii isolates

Author Fernandes, Geisa Ferreira Autor UNIFESP Google Scholar
Amaral, Cristiane Candida do Autor UNIFESP Google Scholar
Sasaki, Alexandre Autor UNIFESP Google Scholar
Godoy, Patricio Martinez Autor UNIFESP Google Scholar
Camargo, Zoilo Pires de Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Austral Chile
Abstract The profiles of proteins present in the exoantigens of Brazilian Sporothrix schenckii isolates were studied and compared by electrophoresis (SDS-PAGE). Thirteen isolates from five different regions of Brazil (1,000 to 2,000 km apart) and ten from a more limited region (200 to 400 km apart within the state of São Paulo) were cultured in Sabouraud, M199 and minimum (MM) media. Qualitative and quantitative differences in the expression of proteins, which varied according to the medium and the isolate, were observed. Fractions with the same MW but varying in intensity were detected, as well as fractions present in 1 isolate but absent in others. Dendrograms were constructed and isolates grouped based on the fractions obtained, irrespective of the intensity. the results showed that Brazilian S. schenckii isolates express different protein profiles, a feature also present in isolates from a more restricted region. the exoantigens were found to have a maximum of 15 protein fractions, ranging in MW from 19-220 KDaltons depending on the medium used for the cultures. These data show the great heterogeneity of Brazilian S. schenckii protein expression.
Keywords Sporothrix schenckii
exoantigens
proteins
SDS-PAGE
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2009-01-01
Published in Medical Mycology. Abingdon: Taylor & Francis Ltd, v. 47, n. 8, p. 855-861, 2009.
ISSN 1369-3786 (Sherpa/Romeo, impact factor)
Publisher Taylor & Francis Ltd
Extent 855-861
Origin http://dx.doi.org/10.3109/13693780802713216
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000273003600010
URI http://repositorio.unifesp.br/handle/11600/31137

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