Neuromodulatory effect of creatine on extracellular action potentials in rat hippocampus: Role of NMDA receptors

Neuromodulatory effect of creatine on extracellular action potentials in rat hippocampus: Role of NMDA receptors

Author Freire Royes, Luiz Fernando Google Scholar
Fighera, Michele Rechia Google Scholar
Furian, Ana Flavia Google Scholar
Oliveira, Mauro Schneider Google Scholar
Fiorenza, Natalia Gindri Google Scholar
Ferreira, Juliano Google Scholar
Silva, Andre Cesar da Autor UNIFESP Google Scholar
Priel, Margareth Rose Autor UNIFESP Google Scholar
Ueda, Erika Sayuri Autor UNIFESP Google Scholar
Calixto, Joao Batista Google Scholar
Cavalheiro, Esper Abrao Autor UNIFESP Google Scholar
Mello, Carlos Fernando Google Scholar
Institution Universidade Federal de Sergipe (UFS)
Univ Fed Rio Grande do Sul
Universidade Federal de São Paulo (UNIFESP)
Universidade Federal de Santa Catarina (UFSC)
Abstract The creatine (Cr) and phosphocreatine (PCr) system is essential for the buffering and transport of high-energy phosphates. Although achievements made over the last years have highlighted the important role of creatine in several neurological diseases, the adaptive processes elicited by this guanidino compound in hippocampus are poorly understood. in the present study, we showed that creatine (0.5-25 mM) gradually increases the amplitude of first population spike (PS) and elicits secondary PS in stratum radiatum of the CA1 region, in hippocampal slices. Creatine also decreased the intensity of the stimulus to induce PS, when compared with hippocampal slices perfused with artificial cerebrospinal fluid (ACSF). the competitive NMDA receptor antagonist, 2-amino-5-phosphonopentanoic acid (AP5; 100 mu M) attenuated creatine-induced increase of amplitude of PS and appearance of secondary PS, providing pharmacological evidence of the involvement of NMDA receptors in the electrophysiological effects of creatine. Accordingly, creatine (0.01-1 mM) increased [H-3]MK-801 binding to hippocampal membranes by 55%, further indicating that this compound modulates NMDA receptor function. These results implicate the NMDA receptor in amplitude and population spike increase elicited by creatine in hippocampus. Furthermore, these data suggest that this guanidino compound may also play a putative role as a neuromodulator in the brain, and that at least some of its effects may be mediated by an increase in glutamatergic function. (c) 2008 Elsevier B.V. All rights reserved.
Keywords creatine
Language English
Date 2008-07-01
Published in Neurochemistry International. Oxford: Pergamon-Elsevier B.V., v. 53, n. 1-2, p. 33-37, 2008.
ISSN 0197-0186 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 33-37
Access rights Closed access
Type Article
Web of Science ID WOS:000258016800005

Show full item record


File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)




My Account