Performance of two-dimensional Doppler echocardiography for the assessment of infarct size and left ventricular function in rats

Performance of two-dimensional Doppler echocardiography for the assessment of infarct size and left ventricular function in rats

Author Nozawa, E. Autor UNIFESP Google Scholar
Kanashiro-Takeuchi, Rosimeire Miyuki Autor UNIFESP Google Scholar
Murad, N. Autor UNIFESP Google Scholar
Carvalho, Antonio Carlos Autor UNIFESP Google Scholar
Cravo, Sergio Luiz Autor UNIFESP Google Scholar
Campos Filho, Orlando Autor UNIFESP Google Scholar
Tucci, Paulo José Ferreira Autor UNIFESP Google Scholar
Moisés, Valdir Ambrósio Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Although echocardiography has been used in rats, few studies have determined its efficacy for estimating myocardial infarct size. Our objective was to estimate the myocardial infarct size, and to evaluate anatomic and functional variables of the left ventricle. Myocardial infarction was produced in 43 female Wistar rats by ligature of the left coronary artery. Echocardiography was performed 5 weeks later to measure left ventricular diameter and transverse area (mean of 3 transverse planes), infarct size (percentage of the arc with infarct on 3 transverse planes), systolic function by the change in fractional area, and diastolic function by mitral inflow parameters. The histologic measurement of myocardial infarction size was similar to the echocardiographic method. Myocardial infarct size ranged from 4.8 to 66.6% when determined by histology and from 5 to 69.8% when determined by echocardiography, with good correlation (r = 0.88; P < 0.05; Pearson correlation coefficient). Left ventricular diameter and mean diastolic transverse area correlated with myocardial infarct size by histology (r = 0.57 and r = 0.78; P < 0.0005). The fractional area change ranged from 28.5 ± 5.6 (large-size myocardial infarction) to 53.1 ± 1.5% (control) and correlated with myocardial infarct size by echocardiography (r = -0.87; P < 0.00001) and histology (r = -0.78; P < 00001). The E/A wave ratio of mitral inflow velocity for animals with large-size myocardial infarction (5.6 ± 2.7) was significantly higher than for all others (control: 1.9 ± 0.1; small-size myocardial infarction: 1.9 ± 0.4; moderate-size myocardial infarction: 2.8 ± 2.3). There was good agreement between echocardiographic and histologic estimates of myocardial infarct size in rats.
Keywords Rats
Myocardial infarction size
Systolic and diastolic function
Doppler echocardiography
Language English
Date 2006-05-01
Published in Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 39, n. 5, p. 687-695, 2006.
ISSN 0100-879X (Sherpa/Romeo, impact factor)
Publisher Associação Brasileira de Divulgação Científica
Extent 687-695
Origin http://dx.doi.org/10.1590/S0100-879X2006000500016
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000237790100016
SciELO ID S0100-879X2006000500016 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/3056

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