Usefulness of a quick decalcification of bone sections embedded in methyl metacrylate: an improved method for immunohistochemistry

Usefulness of a quick decalcification of bone sections embedded in methyl metacrylate: an improved method for immunohistochemistry

Author Gomes, Samirah Abreu Autor UNIFESP Google Scholar
Reis, Luciene Machado dos Google Scholar
Oliveira, Ivone Braga de Google Scholar
Noronha, Irene de Lourdes Google Scholar
Jorgetti, Vanda Google Scholar
Heilberg, Ita Pfeferman Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract Immunohistochemistry of undecalcified bone sections embedded in methyl methacrylate (MMA) is not commonly employed because of potential destruction of tissue antigenicity by highly exothermic polymerization. the aim of the present study was to describe a new technique in which a quick decalcification of bone sections embedded in MMA improves the results for immunohistochemistry. the quality of interleukin 1 alpha (IL-1 alpha) immunostaining according to the present method was better than the conventional one. Immunostaining for osteoprotegerin (OPG) and the receptor activator of NF-kappa B ligand (RANKL) in bone sections of chronic kidney disease patients with mineral bone disorders (CKD-MBD) was stronger than in controls (postmortem healthy subjects). the present study suggested that this method is easy, fast, and effective to perform both histomorphometry and immunohistochemistry in the same bone fragment, yielding new insights into pathophysiological aspects and therapeutic approaches in bone disease.
Keywords bone
immunohistochemistry
methyl methacrylate
osteoprotegerin
chronic kidney disease
Language English
Date 2008-01-01
Published in Journal of Bone and Mineral Metabolism. Tokyo: Springer Tokyo, v. 26, n. 1, p. 110-113, 2008.
ISSN 0914-8779 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 110-113
Origin http://dx.doi.org/10.1007/s00774-007-0788-2
Access rights Closed access
Type Article
Web of Science ID WOS:000251794800017
URI http://repositorio.unifesp.br/handle/11600/30267

Show full item record




File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account