17 beta-estradiol induces the translocation of the estrogen receptors ESR1 and ESR2 to the cell membrane, MAPK3/1 phosphorylation and proliferation of cultured immature rat Sertoli cells

17 beta-estradiol induces the translocation of the estrogen receptors ESR1 and ESR2 to the cell membrane, MAPK3/1 phosphorylation and proliferation of cultured immature rat Sertoli cells

Autor Lucas, Thais F. G. Autor UNIFESP Google Scholar
Siu, Erica R. Autor UNIFESP Google Scholar
Esteves, Carlos A. Autor UNIFESP Google Scholar
Monteiro, Hugo P. Autor UNIFESP Google Scholar
Oliveira, Cleida A. Google Scholar
Porto, Catarina S. Autor UNIFESP Google Scholar
Lazari, Maria Fatima M. Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Universidade Federal de Minas Gerais (UFMG)
Resumo The aim of the present study was to determine the mechanisms involved in estrogen actions in cultured rat Sertoli cells. RT-PCR detected transcripts for the estrogen receptors ESR1 and ESR2 in cultured immature Sertoli cells and in the testis of 15-, 28-, and 120-day-old rats. the expression of ESR1 and ESR2 was confirmed in Sertoli cells by immunofluorescence and Western blot. Immunohistochemistry with cryosections of testes from immature and adult rats revealed that ESR1 is present in Sertoli, Leydig, and some peritubular myoid cells, and ESR2 is present in multiple cell types, including germ cells. Treatment of Sertoli cells with 17beta-estradiol (E-2) induced a translocation of ESR1 and ESR2 to the plasma membrane and a, concomitant phosphorylation of MAPK3/1. Both effects reached a maximum after 10 min and were blocked by PP2, an inhibitor of the SRC family of protein tyrosine kinases, and by the antiestrogen ICI 182,780 (10). MAPK3/1 phosphorylation was also decreased in the presence of AG 1478, an inhibitor of the epidermal growth factor receptor (EGFR) kinase, and in the presence of MAP2K1/2 inhibitor UO126. Treatment with 2 for 24 h increased the incorporation of [methyl-H-3]thymidine, which was blocked by ICI. These results indicate that E2 activates an SRC-mediated translocation of estrogen receptors to the plasma membrane, which results in the activation of EGFR and the mitogen-activated protein kinase signaling pathway. in addition, activation of ESR1 and/or ESR2 by E 2 is involved in proliferation of immature Sertoli cells. the estrogen actions in Sertoli cells might be a key step mediating cellular events important for spermatogenesis and fertility.
Palavra-chave estradiol
estradiol receptor
kinases
mechanisms of hormone action
Sertoli cells
Idioma Inglês
Data de publicação 2008-01-01
Publicado em Biology of Reproduction. Madison: Soc Study Reproduction, v. 78, n. 1, p. 101-114, 2008.
ISSN 0006-3363 (Sherpa/Romeo, fator de impacto)
Publicador Soc Study Reproduction
Extensão 101-114
Fonte http://dx.doi.org/10.1095/biolreprod.107.063909
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000251916000012
Endereço permanente http://repositorio.unifesp.br/handle/11600/30227

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