Placental glutathione S-transferase correlates with cellular proliferation during rat tonguie carcinogenesis induced by 4-nitroquinoline 1-oxide

Placental glutathione S-transferase correlates with cellular proliferation during rat tonguie carcinogenesis induced by 4-nitroquinoline 1-oxide

Author Silva, Renata N. Google Scholar
Ribeiro, Daniel A. Google Scholar
Salvadori, Daisy M. F. Google Scholar
Marques, Mariangela E. A. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Nacl Estadual São Paulo
Abstract Taking into consideration that glutatione S-transferase (GST) and cellular proliferation play a crucial role during carcinogenesis, the goal of this study was to investigate the expression of placental GST, called GST-P, and proliferating cellular nuclear antigen (PCNA) by means of immunohistochemistry during rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide (4NQO). This is a useful model for studying oral squamous cell carcinoma phase by phase. Male Wistar rats were distributed into three groups of 10 animals each and treated with 50 ppm 4NQO solution by drinking water for 4, 12 or 20 weeks. Ten animals were used as negative control. GST-P positive foci were detected in non-neoplastic oral cells at 4 weeks of 4NQO administration. in the same way, GST-P positive cells were detected in pre-neoplastic lesions and squamous cell carcinomas induced after 12 and 20 weeks-treatment, respectively. None of the control animals expressed GST-P positive cells. Regarding cellular proliferation, PCNA positive nuclei were higher at 12 and 20 weeks following 4NQO exposure (p < 0.05) when compared to negative control. These results suggest that the expression of GST-P is correlated with cellular proliferation, in which GST-P is associated with risk and progression of oral cancer, whereas PCNA is closely involved during neoplastic conversion. (c) 2007 Published by Elsevier GmbH.
Keywords placental glutathione S-transferase
Rat tongue mucosa
Oral squamous cell carcinoma
4-nitroquinoline 1-oxide
Language English
Date 2007-09-01
Published in Experimental and Toxicologic Pathology. Jena: Elsevier Gmbh, Urban & Fischer Verlag, v. 59, n. 1, p. 61-68, 2007.
ISSN 0940-2993 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 61-68
Origin http://dx.doi.org/10.1016/j.etp.2007.02.010
Access rights Closed access
Type Article
Web of Science ID WOS:000249608100008
URI http://repositorio.unifesp.br/handle/11600/30005

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