Heparanase expression in circulating lymphocytes of breast cancer patients depends on the presence of the primary tumor and/or systemic metastasis

Heparanase expression in circulating lymphocytes of breast cancer patients depends on the presence of the primary tumor and/or systemic metastasis

Author Theodoro, Therese Rachell Google Scholar
Matos, Leandro Luongo de Autor UNIFESP Google Scholar
Sant'Anna, Aleksandra Vanessa Lambiasi Google Scholar
Fonseca, Fernando Luiz Affonso Autor UNIFESP Google Scholar
Semedo, Patricia Autor UNIFESP Google Scholar
Martins, Lourdes Conceicao Google Scholar
Nader, Helena Bonciani Autor UNIFESP Google Scholar
Del Giglio, Auro Google Scholar
Pinhal, Maria Aparecida da Silva Autor UNIFESP Google Scholar
Institution Fac Med ABC
Universidade Federal de São Paulo (UNIFESP)
Abstract Heparanase is an endo-beta-glucuronidase that is capable of degrading heparan sulfate chains of proteoglycans, generating a variety of bioactive molecules such as growth factors and chemotactic and angiogenic agents. the expression of heparanase was investigated in the peripheral blood mononuclear cell fraction ( PBMC) of 30 patients with breast cancer and 20 healthy control women by reverse transcription-polymerase chain reaction ( RT-PCR) and immunocytochemistry. PBMC samples from all breast cancer patients at study entry showed the expression of heparanase, whereas no expression was observed for healthy women. Immunocytochemistry analysis demonstrated that heparanase was expressed in lymphocytes of breast cancer PBMC. Throughout follow-up, heparanase expression by RTPCR decreased significantly after surgery in patients treated with neoadjuvant chemotherapy ( P = .002) and after tamoxifen treatment ( P = .040), whereas it increased significantly with the advent of systemic metastasis ( P = .027). in vitro, either serum from breast cancer patients or a medium originated from co-culture experiments of MCF-7 cells and lymphocytes from healthy women stimulated heparanase expression in normal lymphocytes. the results suggest that there is a tumor-inducing effect on heparanase expression by lymphocytes present in the PBMCs of breast cancer patients, which depends, in turn, on the interaction between a tumor and normal lymphocytes.
Keywords heparanase
breast cancer
lymphocytes
tumor markers
metastasis
Language English
Date 2007-06-01
Published in Neoplasia. Ann Arbor: Neoplasia Press, v. 9, n. 6, p. 504-510, 2007.
ISSN 1522-8002 (Sherpa/Romeo, impact factor)
Publisher Neoplasia Press
Extent 504-510
Origin http://dx.doi.org/10.1593/neo.07241
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000247366800007
URI http://repositorio.unifesp.br/handle/11600/29779

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