P2X(7)-induced apoptosis decreases by aging in mice myeloblasts

P2X(7)-induced apoptosis decreases by aging in mice myeloblasts

Author Paredes-Gamero, Edgar Julian Google Scholar
Dreyfuss, Juliana Luporini Google Scholar
Nader, Helena B. Google Scholar
Oshiro, Maria Etsuko Miyamoto Autor UNIFESP Google Scholar
Ferreira, Alice Teixeira Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract In the current study, the ability of ATP to promote apoptosis in myeloblasts at different ages was investigated. We have observed that high concentration of extracellular ATP (> 1 mM), which activates P2X(7) receptor, produced cell shrinkage an increase in the number of events in the sub-G(0)/G(1) region of the cellular cycle and annexin-V/propidium iodide label, which characterizes the apoptotic cell death. in addition, BzATP produced apoptosis, but not ADP and UTP. Gr-1(+) cells express the P2X(7) receptor and oxidized ATP, a specific P2X(7) inhibitor, blocked the ATP-dependent apoptosis. ATP-dependent apoptosis is decreased by aging in myeloblasts of 12 and 22-month-old mice. Furthermore, P2X(7) expression decrease was observed in older mice, explaining apoptosis decrease. This decrease in apoptosis by aging may be related to some diseases in the myelocyte lineage. (c) 2007 Published by Elsevier Inc.
Keywords bone marrow
myeloblasts
apoptosis
aging
P2X(7) receptor
Language English
Date 2007-04-01
Published in Experimental Gerontology. Oxford: Pergamon-Elsevier B.V., v. 42, n. 4, p. 320-326, 2007.
ISSN 0531-5565 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 320-326
Origin http://dx.doi.org/10.1016/j.exger.2006.11.011
Access rights Closed access
Type Article
Web of Science ID WOS:000245478100009
URI http://repositorio.unifesp.br/handle/11600/29627

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