Aberrant methylation in pediatric myelodysplastic syndrome

Aberrant methylation in pediatric myelodysplastic syndrome

Author Vidal, Daniel O. Google Scholar
Paixao, Valeria A. Google Scholar
Brait, Mariana Google Scholar
Souto, Elisabeth X. Google Scholar
Caballero, Otavia L. Google Scholar
Lopes, Luiz Fernando Google Scholar
Vettore, Andre L. Google Scholar
Institution Ludwig Inst Canc Res
Diagnost Amer SA
Mem Sloan Kettering Canc Ctr
Universidade Federal de São Paulo (UNIFESP)
Abstract Background: Aberrant methylation of gene promoter region is responsible for inappropriate gene silencing, and it has been associated to initiation and progression of cancer. Aberrant promoter methylation is frequently observed in adult patients with myelodysplastic syndrome (MDS), but in pediatric patients it has been poorly investigated. Methods: We examined the promoter methylation status of 13 genes in bone marrow cells collected at diagnosis of 21 pediatric patients with MDS (subtype RAEB or RAEB-t). for this analysis, we performed sodium bisulfite treatment of genomic DNA, followed by methylation specific PCR (MSP). Results: in pediatric MDS samples, we observed two genes frequently methylated: CALCA was methylated in 85.7% (18/21) of the analyzed samples and CDKN2B in 50% (6/12). Conclusions: Our findings indicate that CALCA and CDKN2B are frequently methylated in pediatric MDS. It suggests that aberrant methylation in pediatric MDS seems to be similar to adult MDS, thus pediatric patients could be also benefited with treatment using demethylating agents. (c) 2006 Elsevier B.V. All fights reserved.
Keywords myelodysplastic syndrome
methylation
MSP
pediatric patients
Language English
Date 2007-02-01
Published in Leukemia Research. Oxford: Pergamon-Elsevier B.V., v. 31, n. 2, p. 175-181, 2007.
ISSN 0145-2126 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 175-181
Origin http://dx.doi.org/10.1016/j.leukres.2006.06.013
Access rights Closed access
Type Article
Web of Science ID WOS:000243631300009
URI http://repositorio.unifesp.br/handle/11600/29496

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