Inhibition of eukaryotic translation initiation factor 5A (eIF5A) hypusination impairs melanoma growth

Inhibition of eukaryotic translation initiation factor 5A (eIF5A) hypusination impairs melanoma growth

Author Jasiulionis, Miriam G. Google Scholar
Luchessi, Augusto D. Google Scholar
Moreira, Andreia G. Google Scholar
Souza, Pedro P. C. Google Scholar
Suenaga, Ana P. M. Google Scholar
Correa, Mariangeta Google Scholar
Costa, Carlos A. S. Google Scholar
Curi, Rui Google Scholar
Costa-Neto, Claudio M. Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
UNESP
Abstract The eukaryotic translation initiation factor 5A (eIF5A) undergoes a specific post-translational modification called hypusination. This modification is required for the functionality of this protein. the compound N1-guanyl-1,7-diaminoheptane (GC7) is a potent and selective inhibitor of deoxyhypusine synthase, which catalyses the first step of eIF5A hypusination process. in the present study, the effects of GC7 on cell death were investigated using two cell lines: melan-a murine melanocytes and Tm5 murine melanoma. in vitro treatment with GC7 increased by 3-fold the number of cells presenting DNA fragmentation in Tm5 cells. Exposure to GC7 also decreased viability to both cell lines. This study also describes, for the first time, the in vivo antitumour effect of GC7, as indicated by impaired melanoma growth in C57BL/6 mice. Copyright (c) 2006 John Wiley & Sons, Ltd.
Keywords tumour growth
cell proliferation
melanoma
cytotoxicity
eIF5A
eIF-5A
hypusine
GC7
Language English
Date 2007-01-01
Published in Cell Biochemistry and Function. Chichester: John Wiley & Sons Ltd, v. 25, n. 1, p. 109-114, 2007.
ISSN 0263-6484 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 109-114
Origin http://dx.doi.org/10.1027/cbf.1351
Access rights Closed access
Type Article
Web of Science ID WOS:000243581300012
URI http://repositorio.unifesp.br/handle/11600/29395

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