Activation of P2Y(1) receptor triggers two calcium signaling pathways in bone marrow erythroblasts

Activation of P2Y(1) receptor triggers two calcium signaling pathways in bone marrow erythroblasts

Author Paredes-Gamero, E. J. Google Scholar
Craveiro, R. B. Google Scholar
Pesquero, J. B. Google Scholar
Franca, J. P. Google Scholar
Oshiro, MEM Google Scholar
Ferreira, A. T. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract In this study, we describe the presence of P2 receptor subtypes and Ca2+ signaling in erythroblasts. ATP and ADP produced a biphasic increase of intracellular Ca2+ concentration ([Ca2+],), with an initial transient phase followed by a sustained phase. Reverse transcription polymerase chain reaction (RT-PCR) showed the expression of P2Y(1), P2Y(2) and P2Y(12). the selective P2Y(1) receptor antagonist 2'-deoxy-N-6-methyl-adenosine- 3',5'diphosphate (MRS2179) and the Gi protein inhibitor pertussis toxin blocked Ca2+ increase. the initial transient [Ca2+](i) increase phase was sensitive to the 1,4,5-inositol trisphosphate (IP3) receptor blocker 2-aminoethoxy-diphenylborate (2-APB), while the sustained phase was sensitive to the protein kinase C (PKC) inhibitor 2-[1-(3-dimethylaminopropyl)-1H-indol-3-yl]-3(1H-indol-3-yl)-maleimide (GF109203X) and calcium calmodulin kinase 11 (CaMKII) inhibitor 1-[N,O-bis(5-isoquinolinesulfonyl)-N-methyl-L-tyrosyl]-4-phenylpiperazine (KN-62). in addition, the PKC activator phorbol-12,13-dibutyrate (PDBu) produced increase of [Ca2+](i). Flow cytometry analysis showed the expression of Ca2+-dependent PKC alpha, beta I, gamma and phospho-CaMKII.These results suggest that the activation of the P2Y(1) receptor triggers two different [Ca2+]i increase pathways, one IP3-dependent and the other kinase-dependent. (c) 2006 Elsevier B.V. All rights reserved.
Keywords bone marrow
erythroblast
P2Y(1)
receptor
intracellular calcium
CaMKII
PKC
Language English
Date 2006-03-18
Published in European Journal of Pharmacology. Amsterdam: Elsevier B.V., v. 534, n. 1-3, p. 30-38, 2006.
ISSN 0014-2999 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 30-38
Origin http://dx.doi.org/10.1016/j.ejphar.2006.01.010
Access rights Closed access
Type Article
Web of Science ID WOS:000236409600004
URI http://repositorio.unifesp.br/handle/11600/28793

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