A bovine protocol for training professionals in preimplantation genetics diagnosis using polymerase chain reactions

A bovine protocol for training professionals in preimplantation genetics diagnosis using polymerase chain reactions

Autor Almodin, Carlos Gilberto Autor UNIFESP Google Scholar
Moron, Antonio Fernandes Autor UNIFESP Google Scholar
Kulay Junior, Luiz Autor UNIFESP Google Scholar
Minguetti-Camara, Vânia Cibele Google Scholar
Moraes, Andréa Cristina Autor UNIFESP Google Scholar
Torloni, Maria Regina Autor UNIFESP Google Scholar
Instituição Materbaby Reprod Humana & Genet
Universidade Federal de São Paulo (UNIFESP)
Resumo Objective: To develop a bovine protocol for training in preimplantation genetic diagnosis (PGD) using PCR.Design: Randomized study.Setting: Human reproduction PCR laboratory.Patient(s): Cow ovaries obtained from slaughterhouses.Intervention(s): the ovaries were punctured and the oocytes were matured and submitted to in vitro fertilization. On the third day after fertilization, the embryos were biopsied and 1-2 blastomeres removed. A blastomere and the rest of the embryo were submitted to PCR for sex determination.Main Outcome Measure(S): Establishment of a possible training protocol.Result(s): A total of 50 embryos and 50 biopsied blastomeres were submitted to DNA amplification for sexing. of the 50 embryos, 41 (82%) achieved successful DNA amplification and 9 (18%) did not. of the 50 biopsies, 31 (62%) amplified and 19 (38%) did not. in 27 (65.9%) of the 41 embryos with DNA amplification, sex was identified as female and in 14 (34.1%) as male. in 40 cases (80%) amplification and sex determination were in both embryos and blastomeres. Sex was identical in all these cases.Conclusion(s): This training model seems to be useful in identifying mistakes and difficulties and improving the professional's performance in the various stages of preimplantation genetic diagnosis.
Assunto PGD
Idioma Inglês
Data 2005-10-01
Publicado em Fertility and Sterility. New York: Elsevier B.V., v. 84, n. 4, p. 895-899, 2005.
ISSN 0015-0282 (Sherpa/Romeo, fator de impacto)
Editor Elsevier B.V.
Extensão 895-899
Fonte http://dx.doi.org/10.1016/j.fertnstert.2005.02.051
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000232542000013
URI http://repositorio.unifesp.br/handle/11600/28474

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