Polystyrene-type resin used for peptide synthesis: application for anion-exchange and affinity chromatography

Polystyrene-type resin used for peptide synthesis: application for anion-exchange and affinity chromatography

Author Carvalho, RSH Google Scholar
Ianzer, D. A. Google Scholar
Malavolta, L. Google Scholar
Rodrigues, M. M. Google Scholar
Cilli, E. M. Google Scholar
Nakaie, C. R. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
UNESP
Abstract This paper deals with an unusual application for a copolymer of styrene-1 % divinylbenzene bearing high amount of aminomethyl groups for anion-exchange and affinity chromatography. the so-called aminomethyl resin (AMR), to date only employed for peptide synthesis, swelled appreciably in water and was used successfully to purify negatively charged peptides. By correlating swelling degree of beads with pH of the media, it was possible to estimate that the AMR amino group pK(a) is approximately 5.5. in addition, the synthesized acetyl-(NANP)(3)-AMR succeeded in the affinity interaction with large antibody molecules related to malaria transmission and raised previously against this dodecapeptide sequence. (C) 2004 Elsevier B.V. All rights reserved.
Keywords resin
peptide
polymer
ion-exchange chromatography
affinity chromatography
Language English
Date 2005-03-25
Published in Journal of Chromatography B-analytical Technologies in the Biomedical and Life Sciences. Amsterdam: Elsevier B.V., v. 817, n. 2, p. 231-238, 2005.
ISSN 1570-0232 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 231-238
Origin http://dx.doi.org/10.1016/j.jchromb.2004.12.008
Access rights Closed access
Type Article
Web of Science ID WOS:000226957700014
URI http://repositorio.unifesp.br/handle/11600/28198

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