Occurrence of insertion sequences within the genomes and Tn1546-like elements of glycopeptide-resistant enterococci isolated in Brazil, and identification of a novel element, ISEfa5

Occurrence of insertion sequences within the genomes and Tn1546-like elements of glycopeptide-resistant enterococci isolated in Brazil, and identification of a novel element, ISEfa5

Author Camargo, ILBC Google Scholar
Zanella, R. C. Google Scholar
Brandileone, MCC Google Scholar
Pignatari, ACC Google Scholar
Goldman, G. H. Google Scholar
Woodford, N. Google Scholar
Lucia, A. Google Scholar
Darini, C. Google Scholar
Institution Universidade de São Paulo (USP)
Inst Adolfo Lutz Registro
Universidade Federal de São Paulo (UNIFESP)
Hlth Protect Agcy
Abstract Insertion sequences (IS) occur widely within the Tn1546-like elements responsible for VanA glycopeptide resistance in enterococci from several countries. As such insertions can be used as epidemiological markers and for studying horizontal transfer of gene clusters, we investigated the distribution of IS6770, IS1542, IS1216V, IS1476, and IS1251 elements in 26 VanA Enterococcus faecium and 21 VanA Enterococcus faecalis from Brazil. PCR, using genomic DNA as a template, indicated that most of the isolates contained IS6770 (97%), IS1216V (87%) and IS1476 (72%) elements. IS1251 was also detected, but at a higher frequency in E faecium (80%) than in E faecalis (14%). None of the isolates harboured IS1542. Only two of 47 isolates had IS elements within their Tn1546-like elements; one possessed IS1251 between vanS and vanH, as reported in the United States; another possessed a novel IS element, designated ISEfa5, located between vanX and van Y. This novel element was found in the genomic DNA of 25 (96%) E faecium and 11 (52%) E faecalis. in stability studies, no IS-mediated changes were detected in the Tn.1546-like elements of 25 vancomycin-resistant enterococci (VRE) monitored over 11 months. These results suggest that the occurrence of IS in Brazilian isolates is similar to that reported in American isolates, but that these elements occur rarely within the vanA gene clusters. As patterns of IS carriage did not correlate with the PFGE type of the VRE, the prevalence of IS elements in genomic DNA of VRE is not a useful epidemiological marker. However, the presence of IS-modified Tn1546-like elements, which appear to be rare in Brazil, could be a useful molecular marker in local epidemiological studies to monitor the evolution and horizontal transmission of VanA elements. (c) 2004 Published by Elsevier GmbH.
Keywords ISEfa5
IS elements
VRE
Enterococcus faecium
Enterococcus faecalis
glycopeptide resistance
Language English
Date 2005-03-01
Published in International Journal of Medical Microbiology. Jena: Urban & Fischer Verlag, v. 294, n. 8, p. 513-519, 2005.
ISSN 1438-4221 (Sherpa/Romeo, impact factor)
Publisher Urban & Fischer Verlag
Extent 513-519
Origin http://dx.doi.org/10.1016/j.ijmm.2004.11.003
Access rights Closed access
Type Article
Web of Science ID WOS:000227950500006
URI http://repositorio.unifesp.br/handle/11600/28178

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