Cyclooxygenase 1 and/or 2 blockade ameliorates the renal tissue damage triggered by ischemia and reperfusion injury

Cyclooxygenase 1 and/or 2 blockade ameliorates the renal tissue damage triggered by ischemia and reperfusion injury

Author Feitoza, C. Q. Google Scholar
Camara, NOS Google Scholar
Pinheiro, H. S. Google Scholar
Goncalves, G. M. Google Scholar
Cenedeze, M. A. Google Scholar
Pacheco-Silva, A. Google Scholar
Santos, OFP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Ischemia-reperfusion injury (IRI) is a common event in organ transplantation. being implicated as a potential contributor for the development of chronic allograft nephropathy. There are new evidences showing a tissue inflammatory response following renal IRI Cyclooxygenases (COX) 1 and 2 can be detected in tissue submitted to IRI and may have impact on organ function outcome. We evaluated the role of COX inhibition on the renal tissue damage that follows M. Mice were submitted to 45 min of renal pedicle ligature and allowed to reperfuse for 24, 48, 72 and 120 h. Blood and kidney samples were collected at reperfusion times. mRNA was extracted from the kidney samples to amplify, COX-1: COX-2 and beta-actin genes. Animals were pretreated with indomethacin or rofecoxib before the surgery. Indomethacin treatment induced a better renal function (serum urea) when compared to control animals at 24, 48 and 72 h (219 +/- 54.5 vs. 338 +/- 51 mg/dl: 106 +/- 51 vs. 326 +/- 86 mg/dl; 94 +/- 14 vs. 138 +/- 38 mg/dl, respectively). Surprisingly, rofecoxib use was associated with even better renal improvement following IR. Animals treated with the later drug showed lower urea values at 24 h post reperfusion compared to indomethacin-treated animals (128 +/- 33 vs. 219 +/- 54.5 mg/dl, P < 0.05). Blockade of COX-1 and -2 resulted in a decrease of tubular necrosis. mRNA COX-2 was up-regulated post IRI and considerable inhibited after indomethacin or rofecoxib treatment. Our data show COX-1/-2 participates in the inflammatory tissue response to IR injury and its inhibition is associated with an improvement in renal function. (C) 2004 Elsevier B.V. All rights reserved.
Keywords ischemia and reperfusion injury
acute renal failure
Language English
Date 2005-01-01
Published in International Immunopharmacology. Amsterdam: Elsevier B.V., v. 5, n. 1, p. 79-84, 2005.
ISSN 1567-5769 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 79-84
Access rights Closed access
Type Article
Web of Science ID WOS:000226615400011

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