Bax affects intracellular Ca2+ stores and induces Ca2+ wave propagation

Bax affects intracellular Ca2+ stores and induces Ca2+ wave propagation

Author Carvalho, ACP Google Scholar
Sharpe, J. Google Scholar
Rosenstock, T. R. Google Scholar
Teles, AFV Google Scholar
Youle, R. J. Google Scholar
Smaili, Soraya Soubhi Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
NINDS
Universidade de São Paulo (USP)
Abstract In the present study, we evaluated proapoptotic protein Bax on mitochondria and Ca2+ homeostasis in primary cultured astrocytes. We found that recombinant Bax (rBax, 10 and 100 ng/ml) induces a loss in mitochondrial membrane potential (DeltaPsi(m)). This effect might be related to the inhibition of respiratory rates and a partial release of cytochrome c, which may change mitochondrial morphology. the loss of DeltaPsi(m) and a selective permeabilization of mitochondrial membranes contribute to the release of Ca2+ from the mitochondria. This was inhibited by cyclosporin A (5 muM) and Ruthenium Red (1 mug/ml), indicating the involvement of mitochondrial Ca2+ transport mechanisms. Bax-induced mitochondrial Ca2+ release evokes Ca2+ waves and wave propagation between cells. Our results show that Bax induces mitochondrial alteration that affects Ca2+ homeostasis and signaling. These changes show that Ca2+ signals might be correlated with the proapoptotic activities of Bax.
Keywords Bax
calcium signaling
mitochondria
endoplasmic reticulum
calcium waves
wave propagation
apoptosis
Language English
Date 2004-12-01
Published in Cell Death and Differentiation. London: Nature Publishing Group, v. 11, n. 12, p. 1265-1276, 2004.
ISSN 1350-9047 (Sherpa/Romeo, impact factor)
Publisher Nature Publishing Group
Extent 1265-1276
Origin http://dx.doi.org/10.1038/sj.cdd.4401508
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000225109800004
URI http://repositorio.unifesp.br/handle/11600/28023

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