Phenotypic modulation of fibroblastic cells in mice pubic symphysis during pregnancy, partum and postpartum

Phenotypic modulation of fibroblastic cells in mice pubic symphysis during pregnancy, partum and postpartum

Author Moraes, S. G. Google Scholar
Pinheiro, M. C. Google Scholar
Toledo, OMS Google Scholar
Joazeiro, P. P. Google Scholar
Institution Universidade Estadual de Campinas (UNICAMP)
Universidade Federal de São Paulo (UNIFESP)
Abstract In many species, the cartilaginous pubic symphysis of the pregnant female is gradually replaced by a fibrous connective tissue, forming a flexible and elastic interpubic ligament. This newly formed ligament is responsible for the separation of the pubic bones, enabling safe delivery of the young. Following labor, the ligament undergoes rapid involution. To our knowledge, no previous work has focused on the phenotypic modulation that is responsible for the changes present at the interpubic ligament throughout the relaxation and closing of the symphysis. the purpose of this study was to investigate the ultrastructural features and immunophenotype of the peculiar cell type found in the pubic symphysis of cycling, pregnant and postpartum mice. in particular, immunohistochemistry studies were conducted on the expressions of the cytoskeletal proteins desmin, vimentin and alpha-smooth muscle actin (alpha-SMA). During pregnancy, the pubic symphysis cells always expressed alpha-SMA, whereas the expression of vimentin and desmin was transient from early pregnancy to postpartum. Furthermore, the expression patterns of these three cytoskeletal proteins were distinct. Cells present in the medial region of the mouse symphysis in cycling and at D12 displayed ultrastructural features characteristic of a typical fibroblast. in contrast, during the last week of pregnancy and in postpartum these cells acquired ultrastructural features representative of a myofibroblast; for example, a fibronexus and a contractile apparatus were found to be present lying in close contact with the extracellular collagenous and elastic system fibrils. Taken together, these results strongly suggest a contractile function for these cells which might contribute to support of the varying mechanical stresses present during pubic bone movement.
Keywords pubic symphysis
pregnancy
immunohistochemistry
desmin
vimentin
alpha-SMA
mouse (Swiss)
Language English
Date 2004-02-01
Published in Cell and Tissue Research. New York: Springer-verlag, v. 315, n. 2, p. 223-231, 2004.
ISSN 0302-766X (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 223-231
Origin http://dx.doi.org/10.1007/s00441-003-0826-x
Access rights Closed access
Type Article
Web of Science ID WOS:000188493400009
URI http://repositorio.unifesp.br/handle/11600/27603

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