Use of the sensitive/less-sensitive (detuned) EIA strategy for targeting genetic analysis of HIV-1 to recently infected blood donors

Use of the sensitive/less-sensitive (detuned) EIA strategy for targeting genetic analysis of HIV-1 to recently infected blood donors

Author Machado, Daisy M. [UNIFESP} Google Scholar
Delwart, E. L. Google Scholar
Diaz, Ricardo Sobhie Autor UNIFESP Google Scholar
Oliveira, Carlos F. de Autor UNIFESP Google Scholar
Alves, Katia Autor UNIFESP Google Scholar
Rawal, B. D. Google Scholar
Sullivan, M. Google Scholar
Gwinn, M. Google Scholar
Clark, K. A. Google Scholar
Busch, M. P. Google Scholar
Institution Blood Ctr Pacific
Universidade Federal de São Paulo (UNIFESP)
Univ Calif San Francisco
Natl Blood Data Resource Ctr
Ctr Dis Control & Prevent
Abstract Objective: To corroborate the validity of the recently developed sensitive/less sensitive (S/LS) dual enzyme immunoassay (EIA) strategy for the detection of recently infected individuals and to genetically analyze recently transmitted strains of HIV-1 in a US blood donor population.Design: the S/LS EIA strategy was used to identify 33 recently infected subjects among 281 enrolled HIV-1 seropositive blood donors (from a total of 410 HIV-1 infected subjects identified from 5 230 463 blood donations screened by participating US blood centers in 1995-1996)Methods: We analysed three host response and viral characteristics were associated with recent HIV-1 infection: rapidly increasing EIA optical density (OD) values, genetically homogeneous env gene quasispecies, and putative non-syncytium inducing env V3 loop sequences. the drug resistance genotypes of the recently transmitted strains were determined by DNA sequencing.Results: Increasing EIA OD values, clonal HIV-1 quasispecies and V3 loop sequences with inferred NSI phenotypes were generally detected in LS EIA non-reactive samples, Thirty-two subtype B and one CRF02_AG recombinant HIV-1 were detected. Genetic evidence for drug resistance to zidovudine (K70R) and non-nucleoside analog reverse transcriptase inhibitors (VI 081) was detected in one strain each, and three other strains showed the presence of accessory protease inhibitor resistance mutations.Conclusions: Immunologic and virologic results further substantiate the validity of the S/LS EIA strategy for the detection of recent infections and illustrate its use for targeting molecular and epidemiological investigations to incident cases identified from large cross-sectional screening programs, rather than the more costly and logistically difficult longitudinal studies. (C) 2002 Lippincott Williams Wilkins.
Keywords acute infection
HIV diagnostic tests
HIV sequence variability
detuned EIA
Language English
Date 2002-01-04
Published in Aids. Philadelphia: Lippincott Williams & Wilkins, v. 16, n. 1, p. 113-119, 2002.
ISSN 0269-9370 (Sherpa/Romeo, impact factor)
Publisher Lippincott Williams & Wilkins
Extent 113-119
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000173225800014

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