Portal hypertensive response to bradykinin in inflamed or cirrhotic rat livers is mediated by B-2-type receptors

Portal hypertensive response to bradykinin in inflamed or cirrhotic rat livers is mediated by B-2-type receptors

Author Loureiro-Silva, M. R. Google Scholar
Molina, H. M. Google Scholar
Borges, D. R. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Background: We have shown that the portal hypertensive response to bradykinin in normal rats is mediated by B-2 receptors.Methods: By using isolated and exsanguinated rat liver perfusion, we studied the portal hypertensive response to bradykinin or des-Arg(9)-bradykinin (B-1 agonist) in inflamed or cirrhotic rat livers. Livers were perfused with bovine serum albumin Krebs-Henseleit buffer (pH 7.4; 37 degreesC) at a constant flow rate, in the absence or presence of des-Arg(9)[Leu(8)]-bradykinin or HOE 140 (B-1 and B-2 receptor antagonists, respectively). Bradykinin (140 nmol) or des-Arg(9)-bradykinin was injected as a bolus via the afferent route to the liver.Results: Basal perfusion pressure in liver-cirrhotic rats was higher than in normal rats. in normal, inflamed, or liver-cirrhotic rats, the presence of the B-1 antagonist did not change the portal hypertensive response to bradykinin, while the B-2 antagonist abolished this response. A 140-nmol dose of des-Arg(9)-bradykinin did not change the perfusion pressure; 700 nmol of this B-1 agonist produced an insignificant perfusion pressure increase. the perfusion pressure increase induced by bradykinin in cirrhotic livers was lower than in normal livers.Conclusions: the portal hypertensive response to bradykinin in inflamed or cirrhotic rat livers is mediated by B-2 receptors, but not B-1 receptors, and there is a contracting hyporeactivity to bradykinin in cirrhotic rat livers. (C) 2001 Blackwell Science Asia Pty Ltd.
Keywords acute phase response
bradykinin antagonist
bradykinin receptor
bradykinin
cirrhosis
des-Arg(9)-bradykinin
portal hypertension
rat liver perfusion
Language English
Date 2001-01-01
Published in Journal of Gastroenterology and Hepatology. Carlton: Blackwell Science Asia, v. 16, n. 1, p. 41-45, 2001.
ISSN 0815-9319 (Sherpa/Romeo, impact factor)
Publisher Blackwell Science Asia
Extent 41-45
Origin http://dx.doi.org/10.1046/j.1440-1746.2001.02397.x
Access rights Closed access
Type Article
Web of Science ID WOS:000167674800007
URI http://repositorio.unifesp.br/handle/11600/26453

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