Distribution of sulfated glycosaminoglycans in the animal kingdom: widespread occurrence of heparin-like compounds in invertebrates

Distribution of sulfated glycosaminoglycans in the animal kingdom: widespread occurrence of heparin-like compounds in invertebrates

Author Medeiros, G. F. Google Scholar
Mendes, A. Google Scholar
Castro, RAB Google Scholar
Bau, E. C. Google Scholar
Nader, H. B. Google Scholar
Dietrich, C. P. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Fed Rio Grande Norte
Abstract Sulfated glycosaminoglycans were isolated from 23 species of 13 phyla of invertebrates and characterized by their electrophoretic migration in three different buffer systems coupled with enzymatic degradation using bacterial heparinase, heparitinases and chondroitinase AC. Heparan sulfate is a ubiquitous compound present in all species analyzed whereas chondroitin sulfate was present in 20 species and heparin-like compounds in 12 species of the invertebrates. the heparin-like compounds were purified from the echinoderm Mellita quinquisperforata (sand dollar) and the crustacean Ucides cordatus (crab) with anticoagulant activities of 60 and 52 IU/mg, respectively. Degradation of these heparins with heparinase produced significant amounts of the trisulfated disaccharide typical of mammalian heparins. This was confirmed by C-13-NMR spectroscopy of the crab heparin. An updated phylogenetic tree of the distribution of sulfated glycosaminoglycans in the animal kingdom is also presented. (C) 2000 Elsevier Science B.V. All rights reserved.
Keywords sulfated glycosaminoglycan distribution
sulfated glycosaminoglycan
invertebrate
heparan sulfate
chondroitin sulfate
heparin
Language English
Date 2000-07-26
Published in Biochimica Et Biophysica Acta-general Subjects. Amsterdam: Elsevier B.V., v. 1475, n. 3, p. 287-294, 2000.
ISSN 0304-4165 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 287-294
Origin http://dx.doi.org/10.1016/S0304-4165(00)00079-9
Access rights Closed access
Type Article
Web of Science ID WOS:000088518000012
URI http://repositorio.unifesp.br/handle/11600/26347

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