Autoantibodies to DEK oncoprotein in human inflammatory disease

Autoantibodies to DEK oncoprotein in human inflammatory disease

Author Dong, X. W. Google Scholar
Wang, J. S. Google Scholar
Kabir, F. N. Google Scholar
Shaw, M. Google Scholar
Reed, A. M. Google Scholar
Stein, L. Google Scholar
Andrade, Luiz Eduardo Coelho Autor UNIFESP Google Scholar
Trevisani, Virgínia Fernandes Moça Autor UNIFESP Google Scholar
Miller, M. L. Google Scholar
Fujii, T. Google Scholar
Akizuki, M. Google Scholar
Pachman, L. M. Google Scholar
Satoh, M. Google Scholar
Reeves, W. H. Google Scholar
Institution Univ N Carolina
Universidade Federal de São Paulo (UNIFESP)
Northwestern Univ
Keio Univ Hosp
Abstract Objective. To evaluate the specificity of anti-DEK antibodies for juvenile rheumatoid arthritis (JRA),Methods. Anti-DEK autoantibodies were measured by enzyme-linked immunosorbent assay (ELISA) using affinity-purified his6-DEK fusion protein. Sera from 639 subjects (417 patients with systemic autoimmune disease, 13 with sarcoidosis, 44 with pulmonary tuberculosis, 125 with uveitis, and 6 with scleritis, and 34 healthy control subjects) were screened. Reactivity was verified by immunoblotting and immunoprecipitation Studies using baculovirus-expressed human DEK.Results. Anti-DEK activity was found at the following frequencies: JRA 39.4% (n = 71), systemic lupus erythematosus (SLE) 25.1% (n = 216), sarcoidosis 46.2% (n = 13), rheumatoid arthritis 15.5% (n = 71), systemic sclerosis 36.0% (n = 22), polymyositis 6.2% (n = 16), and adult Still's disease 0% (n = 21), Autoantibodies also were detected in 9.1% of tuberculosis sera (n = 44), but were undetectable in sera from the 34 healthy controls. Western blot and immunoprecipitation assay results correlated well with the ELISA findings. in general, levels of anti-DEK autoantibodies were higher in SLE than in other patient subsets, including JRA.Conclusion. Anti-DEK autoantibodies are less specific for JRA than previously believed. They are produced in association with a variety of inflammatory conditions, many of which are associated with granuloma formation and/or predominant Th1 cytokine production. Anti-DEK antibodies may be a marker for a subset of autoimmunity associated with interferon-gamma production rather than a particular disease subset.
Language English
Date 2000-01-01
Published in Arthritis and Rheumatism. Philadelphia: Lippincott Williams & Wilkins, v. 43, n. 1, p. 85-93, 2000.
ISSN 0004-3591 (Sherpa/Romeo, impact factor)
Publisher Lippincott Williams & Wilkins
Extent 85-93
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000084782700011

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